Saccharomyces cerevisiae Meyen ex E.C. Hansen (budding yeast) [SCE]

FULL NAME: DNA topoisomerase 1


DESCRIPTION:
The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.

MISCELLANEOUS:
In yeast, topoisomerase I seems to be dispensable. This is thought to be due to the abundant presence of topoisomerase II that can substitute for the relaxing activity of topoisomerase I.
When a topoisomerase transiently breaks a DNA backbone bond, it simultaneously forms a protein-DNA link, in which a tyrosyl oxygen in the enzyme is joined to a DNA phosphorus at one end of the enzyme-severed DNA strand.
Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.
Present with 2970 molecules/cell in log phase SD medium.


STRUCTURE SIMILARITY:
Belongs to the eukaryotic type I topoisomerase family.


CATALYTIC ACTIVITY:
ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.


POST-TRANSLATIONAL MODIFICATION:
Sumoylated. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm (By similarity).


RELATED PATHWAY(S):
DNA replication


Amino acids sequence

        10         20         30         40         50         60
MTIADASKVN HELSSDDDDD VPLSQTLKKR KVASMNSASL QDEAEPYDSD EAISKISKKK
        70         80         90        100        110        120
TKKIKTEPVQ SSSLPSPPAK KSATSKPKKI KKEDGDVKVK TTKKEEQENE KKKREEEEEE
       130        140        150        160        170        180
DKKAKEEEEE YKWWEKENED DTIKWVTLKH NGVIFPPPYQ PLPSHIKLYY DGKPVDLPPQ
       190        200        210        220        230        240
AEEVAGFFAA LLESDHAKNP VFQKNFFNDF LQVLKESGGP LNGIEIKEFS RCDFTKMFDY
       250        260        270        280        290        300
FQLQKEQKKQ LTSQEKKQIR LEREKFEEDY KFCELDGRRE QVGNFKVEPP DLFRGRGAHP
       310        320        330        340        350        360
KTGKLKRRVN PEDIVLNLSK DAPVPPAPEG HKWGEIRHDN TVQWLAMWRE NIFNSFKYVR
       370        380        390        400        410        420
LAANSSLKGQ SDYKKFEKAR QLKSYIDAIR RDYTRNLKSK VMLERQKAVA IYLIDVFALR
       430        440        450        460        470        480
AGGEKSEDEA DTVGCCSLRY EHVTLKPPNT VIFDFLGKDS IRFYQEVEVD KQVFKNLTIF
       490        500        510        520        530        540
KRPPKQPGHQ LFDRLDPSIL NKYLQNYMPG LTAKVFRTYN ASKTMQDQLD LIPNKGSVAE
       550        560        570        580        590        600
KILKYNAANR TVAILCNHQR TVTKGHAQTV EKANNRIQEL EWQKIRCKRA ILQLDKDLLK
       610        620        630        640        650        660
KEPKYFEEID DLTKEDEATI HKRIIDREIE KYQRKFVREN DKRKFEKEEL LPESQLKEWL
       670        680        690        700        710        720
EKVDEKKQEF EKELKTGEVE LKSSWNSVEK IKAQVEKLEQ RIQTSSIQLK DKEENSQVSL
       730        740        750        760
GTSKINYIDP RLSVVFCKKY DVPIEKIFTK TLREKFKWAI ESVDENWRF 

Encoded by TOP1 gene


OTHER NAME(S):
MAK1
MAK17


Nucleic acid sequence

        10         20         30         40         50         60
atgactattg ctgatgcttc caaagttaat catgagttgt cttctgatga cgatgacgat
        70         80         90        100        110        120
gtgccattat ctcaaacttt aaaaaaaaga aaggtggcgt ccatgaactc tgcctctctt
       130        140        150        160        170        180
caagacgaag cggaacctta tgatagtgat gaggcaatct ctaagatttc caagaaaaag
       190        200        210        220        230        240
actaagaaaa taaagaccga accagtgcaa tcgtcgtcat taccatcgcc tccagcaaag
       250        260        270        280        290        300
aaaagcgcga catcaaagcc taaaaaaatc aagaaagaag atggtgatgt aaaggtaaaa
       310        320        330        340        350        360
acaactaaaa aggaagaaca ggagaacgaa aaaaagaaac gagaagaaga agaagaggag
       370        380        390        400        410        420
gacaagaaag cgaaggagga ggaggaagaa tataaatggt gggaaaaaga aaacgaagat
       430        440        450        460        470        480
gacaccataa aatgggtcac actgaagcat aacggtgtta tattccctcc accataccag
       490        500        510        520        530        540
cccttaccat ctcacatcaa attatattac gatgggaagc cagtagattt acctccgcaa
       550        560        570        580        590        600
gctgaagaag tagccgggtt ctttgctgcc ctattagaga gtgatcatgc caaaaatcct
       610        620        630        640        650        660
gttttccaaa agaacttctt caatgatttc ttgcaagtac tgaaagaaag tggtggtccc
       670        680        690        700        710        720
ctcaatggaa ttgagataaa ggaattttct cgttgcgatt tcaccaaaat gtttgattac
       730        740        750        760        770        780
ttccagttac aaaaagaaca gaaaaagcaa ctgacttccc aagaaaagaa acagattcgt
       790        800        810        820        830        840
ttggaaagag aaaaattcga ggaagattat aaattctgtg aattagatgg cagaagggaa
       850        860        870        880        890        900
caagtaggga atttcaaggt tgaacctcct gatctattta gaggtcgtgg cgctcaccca
       910        920        930        940        950        960
aaaacaggca aattgaagag aagagtgaat cctgaggata tcgttttaaa tctaagtaaa
       970        980        990       1000       1010       1020
gacgcacccg ttccgccagc cccagaaggg cacaagtggg gtgaaatcag acacgacaat
      1030       1040       1050       1060       1070       1080
accgttcaat ggttagccat gtggagagag aatattttca actcattcaa atacgtcaga
      1090       1100       1110       1120       1130       1140
ttggcagcga actcttcatt gaagggtcaa agtgactaca agaagtttga aaaggcgaga
      1150       1160       1170       1180       1190       1200
caattgaaat cctatatcga tgccatcaga agggattaca cgagaaattt gaaaagcaaa
      1210       1220       1230       1240       1250       1260
gttatgctag agcgccaaaa ggccgtagcc atttatttga tcgatgtatt cgctttaaga
      1270       1280       1290       1300       1310       1320
gccggtggtg aaaaatccga agatgaagcc gatactgtgg gttgttgttc attgcgatat
      1330       1340       1350       1360       1370       1380
gagcatgtta ctttgaaacc tccgaatact gttatctttg atttcttagg taaggattct
      1390       1400       1410       1420       1430       1440
attagatttt atcaagaggt agaagttgac aaacaagttt tcaaaaattt gacaattttt
      1450       1460       1470       1480       1490       1500
aaaaggccgc ccaaacagcc aggacatcaa ctgtttgatc gtctagatcc atctatactg
      1510       1520       1530       1540       1550       1560
aacaaatatc tacaaaacta catgccggga ttgactgcta aagttttccg tacatataat
      1570       1580       1590       1600       1610       1620
gcttccaaaa caatgcaaga tcaactggat ctaattccaa ataaaggatc tgtcgcagag
      1630       1640       1650       1660       1670       1680
aaaatattga agtacaacgc agcaaataga actgtagcca tcctatgtaa ccatcaaagg
      1690       1700       1710       1720       1730       1740
actgtcacga aggggcatgc acaaacagtg gaaaaggcca ataatagaat acaagagttg
      1750       1760       1770       1780       1790       1800
gaatggcaaa agattcgttg caagagggcc attttacaat tggataagga tcttttaaag
      1810       1820       1830       1840       1850       1860
aaagagccaa aatatttcga agaaatcgac gatttgacga aagaagatga agccaccatt
      1870       1880       1890       1900       1910       1920
cacaagagaa ttattgatag agaaattgaa aaatatcagc gaaaatttgt tagggagaac
      1930       1940       1950       1960       1970       1980
gataagagga aatttgaaaa ggaagaatta ttgccggaaa gtcaattgaa ggaatggttg
      1990       2000       2010       2020       2030       2040
gagaaagtcg acgaaaagaa acaagaattc gaaaaggaat tgaaaaccgg tgaagtggaa
      2050       2060       2070       2080       2090       2100
ctgaaatcaa gttggaattc agtcgaaaaa ataaaagcac aagtagagaa attagaacag
      2110       2120       2130       2140       2150       2160
cgtatccaaa ctagttccat tcagttgaaa gataaagagg aaaactccca ggtttcactg
      2170       2180       2190       2200       2210       2220
ggcacttcca aaatcaatta tatagaccct agactttctg tggtattttg caaaaagtat
      2230       2240       2250       2260       2270       2280
gatgttccga ttgaaaagat ttttacaaaa accctaagag aaaaattcaa atgggccata
      2290       2300       2310
gaatcggtag atgaaaattg gaggttttaa   

Last modification date: Oct. 2, 2011