Arabidopsis thaliana (L.) Heynh. (mouse-ear cress) [ATH]

FULL NAME: Cytosine-specific methyltransferase


STRUCTURE SIMILARITY:
Belongs to the C5-methyltransferase family.


CATALYTIC ACTIVITY:
S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.


RELATED PATHWAY(S):
DNA replication
heterochromatin formation


Amino acids sequence

        10         20         30         40         50         60
MEMETKAGKQ KKRSVDSDDD VSKERRPKRA AACTNFKEKS LRISDKSETV EAKKEQILAE
        70         80         90        100        110        120
EIVAIQLTSS LESNDDPRPN RRLTDFVLHD SEGVPQPVEM LELGDIFIEG VVLPLGDEKK
       130        140        150        160        170        180
EEKGVRFQSF GRVENWNISG YEDGSPVIWI STALADYDCR KPSKKYKKLY DYFFEKACAC
       190        200        210        220        230        240
VEVFKSLSKN PDTSLDELLA AVSRSMSGSK IFSSGGAIQE FVISQGEFIY NQLAGLDETA
       250        260        270        280        290        300
KNHETCFVEN RVLVSLRDHE SNKIHKALSN VALRIDESKV VTSDHLVDGA EDEDVKYAKL
       310        320        330        340        350        360
IQEEEYRKSM ERSRNKRSST TSGGSSRFYI KISEDEIADD YPLPSYYKNT KEETDELVLF
       370        380        390        400        410        420
EAGYEVDTRD LPCRTLHNWT LYNSDSRMIS LEVLPMRPCA EIDVTVFGSG VVAEDDGSGF
       430        440        450        460        470        480
CLDDSESSTS TQSNDHDGMN IFLSQIKEWM IEFGAEMIFV TLRTDMAWYR LGKPSKQYAP
       490        500        510        520        530        540
WFGTVMKTVR VGISIFNMLM RESRVAKLSY ANVIKRLCGL EENDKAYISS KLLDVERYVV
       550        560        570        580        590        600
VHGQIILQLF EEYPDKDIKR CPFVTSLASK MQDIHHTKWI IKKKKKILQK GKNLNPRAGI
       610        620        630        640        650        660
APVVSRMKAM QATTTRLVNR IWGEFYSIYS PEVPSEAINA ENVEEEELEE VEEEDENEED
       670        680        690        700        710        720
DPEENELEAV EIQNSPTPKK IKGISEDMEI KWDGEILGKT SAGEPLYGRA FVGGDVVVVG
       730        740        750        760        770        780
SAVILEVDDQ DDTQLICFVE FMFESSNHSK MLHGKLLQRG SETVLGMAAN ERELFLTNEC
       790        800        810        820        830        840
LTVQLKDIKG TVSLEIRSRL WGHQYRKENI DVDKLDRARA EERKTNGLPT DYYCKSLYSP
       850        860        870        880        890        900
ERGGFFSLPR NDMGLGSGFC SSCKIRENEE ERSKTKLNDS KTGFLSNGIE YHNGDFVYVL
       910        920        930        940        950        960
PNYITKDGLK KGSRRTTLKC GRNVGLKAFV VCQLLDVIVL EESRKASKAS FQVKLTRFYR
       970        980        990       1000       1010       1020
PEDISEEKAY ASDIQELYYS QDTYILPPEA IQGKCEVRKK SDMPLCREYP ILDHIFFCEV
      1030       1040       1050       1060       1070       1080
FYDSSTGYLK QFPANMKLKF STIKDETLLR EKKGKGVETG TSSGMLMKPD EVPKEKPLAT
      1090       1100       1110       1120       1130       1140
LDIFAGCGGL SHGLENAGVS TTKWAIEYEE PAGHAFKQNH PEATVFVDNC NVILRAIMEK
      1150       1160       1170       1180       1190       1200
CGDVDDCVST VEAAELAAKL DENQKSTLPL PGQVDFINGG PPCQGFSGMN RFSHGSWSKV
      1210       1220       1230       1240       1250       1260
QCEMILAFLS FADYFRPKYF LLENVKKFVT YNKGRTFQLT MASLLEMGYQ VRFGILEAGT
      1270       1280       1290       1300       1310       1320
YGVSQPRKRV IIWAASPEEV LPEWPEPMHV FDNPGSKISL PRGLRYDAGC NTKFGAPFRS
      1330       1340       1350       1360       1370       1380
ITVRDTIGDL PPVENGESKI NKEYGTTPAS WFQKKIRGNM SVLTDHICKG LNELNLIRCK
      1390       1400       1410       1420       1430       1440
KIPKRPGADW RDLPDENVTL SNGLVEKLRP LALSKTAKNH NEWKGLYGRL DWQGNLPISI
      1450       1460       1470       1480       1490       1500
TDPQPMGKVG MCFHPEQDRI ITVRECARSQ GFPDSYEFSG TTKHKHRQIG NAVPPPLAFA
      1510
LGRKLKEALY LKSSLQHQS    

Encoded by DMT2 gene


OTHER NAME(S):
DL3110W
DMT02
DMT2
DNA METHYLTRANSFERASE 02
DNA methyltransferase 2
DNA METHYLTRANSFERASE 2
FCAALL.111
MET02
MET2


DESCRIPTION:
This gene is predicted to encode a DNA methyltransferase. A plant line expressing an RNAi construct directed against DMT2 has reduced agrobacterium-mediated tumor formation.


Nucleic acid sequence

        10         20         30         40         50         60
atggaaatgg agacaaaagc tggaaagcaa aagaagagaa gtgttgactc ggatgatgat
        70         80         90        100        110        120
gtctctaagg aaaggagacc aaagcgagca gcagcttgca caaacttcaa ggagaaatct
       130        140        150        160        170        180
ctccgtatct ctgacaaatc tgaaactgtt gaagctaaga aagagcagat cttggcggag
       190        200        210        220        230        240
gagatcgtgg ccatacagtt aacttcttct ttggagagca atgatgatcc tcgtccaaac
       250        260        270        280        290        300
cggaggctga ctgatttcgt tttacatgat tcagaaggag ttccacagcc tgtggagatg
       310        320        330        340        350        360
ttggaacttg gtgacatatt tattgaaggt gttgtcttac ctttaggtga tgagaaaaag
       370        380        390        400        410        420
gaagaaaagg gtgtcaggtt tcaatctttt ggtcgagtag agaactggaa tatatctggt
       430        440        450        460        470        480
tatgaagatg gttccccggt gatatggatt tcaacggcgt tagcggatta cgattgccgt
       490        500        510        520        530        540
aaaccttcta agaaatacaa gaaattatat gattatttct ttgagaaagc ttgtgcttgt
       550        560        570        580        590        600
gtggaggtgt ttaagagttt gtccaagaat ccggatacaa gtcttgatga gcttcttgcg
       610        620        630        640        650        660
gcggtttcta ggtcgatgag cggaagcaag atcttttcta gcggtggagc catccaagag
       670        680        690        700        710        720
tttgttatat cccaaggaga attcatatat aaccaactcg ctggattgga tgagacagcc
       730        740        750        760        770        780
aagaatcatg aaacatgctt tgttgagaat cgtgttcttg tttctctaag agaccatgag
       790        800        810        820        830        840
agtaataaaa ttcacaaggc tttgtctaat gtggctctga ggattgatga gagcaaggtc
       850        860        870        880        890        900
gtgacatctg atcatctggt ggatggtgcc gaggacgagg acgtaaaata tgctaagcta
       910        920        930        940        950        960
atccaagaag aagagtatcg gaaatctatg gagcggtcga gaaataagag aagctcaaca
       970        980        990       1000       1010       1020
acttctggtg gttcaagcag gttttacatt aagatcagtg aagatgagat tgccgatgat
      1030       1040       1050       1060       1070       1080
tatcctctcc cgtcttacta taagaacacc aaagaagaaa cagatgagct tgttctcttt
      1090       1100       1110       1120       1130       1140
gaagctggct atgaggttga tacaagagac ctaccttgca gaacactgca taattggact
      1150       1160       1170       1180       1190       1200
ctttacaact ctgattcacg gatgatatct ttagaggttc ttcccatgag gccgtgtgct
      1210       1220       1230       1240       1250       1260
gagatcgatg tcaccgtctt tgggtcaggt gttgttgctg aagatgatgg aagcgggttt
      1270       1280       1290       1300       1310       1320
tgtctcgatg attcggagag ctctacctct acgcaatcaa atgatcatga tgggatgaat
      1330       1340       1350       1360       1370       1380
atattcctta gtcagataaa ggaatggatg attgagtttg gggcagaaat gatctttgtc
      1390       1400       1410       1420       1430       1440
acattacgaa ctgacatggc ctggtaccga cttgggaaac cgtcaaagca atatgctcct
      1450       1460       1470       1480       1490       1500
tggtttggaa ctgttatgaa aacagtaagg gttggcataa gcattttcaa tatgctcatg
      1510       1520       1530       1540       1550       1560
agggaaagta gggttgctaa gctttcatat gcaaatgtca taaaaagact ttgtgggtta
      1570       1580       1590       1600       1610       1620
gaggagaacg ataaagctta catttcttct aagctcttgg atgttgagag atatgttgtc
      1630       1640       1650       1660       1670       1680
gtccatggac aaattatctt gcagcttttc gaagagtatc ctgacaagga tatcaaaagg
      1690       1700       1710       1720       1730       1740
tgtccatttg ttactagtct tgcaagtaaa atgcaggata tacaccacac aaaatggatc
      1750       1760       1770       1780       1790       1800
atcaagaaga agaagaaaat tctgcaaaag ggaaagaatt tgaatccgag ggcggggatt
      1810       1820       1830       1840       1850       1860
gcacctgtgg tatccagaat gaaagctatg caagcaacaa caactcgcct cgtcaatagg
      1870       1880       1890       1900       1910       1920
atttggggag agttttactc catttactct cctgaggttc catcggaggc cattaatgct
      1930       1940       1950       1960       1970       1980
gaaaatgtgg aggaagagga gcttgaagag gtagaagagg aggacgagaa tgaggaagat
      1990       2000       2010       2020       2030       2040
gatccagagg agaatgaact agaagctgtt gagattcaaa attctcctac tcctaagaaa
      2050       2060       2070       2080       2090       2100
attaaaggta tttctgaaga tatggaaata aaatgggatg gtgagattct tggcaaaact
      2110       2120       2130       2140       2150       2160
tctgctggtg agcctctcta tggaagagcc tttgttggag gggatgtggt ggtggtaggt
      2170       2180       2190       2200       2210       2220
agtgcagtca tcttggaagt tgatgatcaa gatgatactc aattgatctg ttttgtggag
      2230       2240       2250       2260       2270       2280
ttcatgttcg agagttcaaa ccacagcaag atgctacatg ggaaactctt acaaagagga
      2290       2300       2310       2320       2330       2340
tctgagactg ttctaggaat ggctgctaac gagagggaac tgttcttgac taatgaatgt
      2350       2360       2370       2380       2390       2400
cttactgtcc agcttaagga cataaaagga acagttagtc tcgagattcg atcaaggctg
      2410       2420       2430       2440       2450       2460
tgggggcatc aatacaggaa agagaacatc gatgtggata agcttgaccg ggcaagagca
      2470       2480       2490       2500       2510       2520
gaagaaagaa aaactaatgg tttgccaaca gactactact gcaaaagttt gtactcacct
      2530       2540       2550       2560       2570       2580
gagagaggtg gattctttag tcttccaaga aatgatatgg gtctaggttc tggattctgt
      2590       2600       2610       2620       2630       2640
agttcatgta agataagaga gaatgaagag gaaaggtcaa aaactaaact caatgattca
      2650       2660       2670       2680       2690       2700
aagacaggat ttctctccaa tgggatagag tatcataatg gagattttgt ctatgtactc
      2710       2720       2730       2740       2750       2760
cccaactaca taactaaaga tggattgaag aagggtagta gaagaacaac tcttaagtgt
      2770       2780       2790       2800       2810       2820
ggtcggaacg ttgggttaaa agcttttgtt gtttgccaat tgctggatgt tattgttcta
      2830       2840       2850       2860       2870       2880
gaagaatcta gaaaagctag taaggcttca tttcaggtta aactgacaag gttttatagg
      2890       2900       2910       2920       2930       2940
cccgaggaca tttcagaaga aaaggcctat gcttcagaca tccaagagtt gtattatagc
      2950       2960       2970       2980       2990       3000
caggatacat atattctccc tccggaagct atacagggaa aatgtgaagt aaggaagaaa
      3010       3020       3030       3040       3050       3060
agtgatatgc ccctatgtcg tgagtatcca attttagacc atatcttctt ctgtgaagtt
      3070       3080       3090       3100       3110       3120
ttctatgatt cctctactgg ttatctcaag cagtttccgg cgaatatgaa gctgaagttc
      3130       3140       3150       3160       3170       3180
tctactatta aagatgaaac acttctaaga gaaaagaagg ggaagggagt agaaactgga
      3190       3200       3210       3220       3230       3240
actagttctg gaatgcttat gaagcctgat gaggtaccta aagagaagcc tctagctaca
      3250       3260       3270       3280       3290       3300
ctagatattt ttgctggatg tggtggtcta tctcatggac tagaaaatgc tggtgtatct
      3310       3320       3330       3340       3350       3360
actacaaagt gggcgatcga gtatgaagag ccagctggtc atgcgtttaa acaaaaccat
      3370       3380       3390       3400       3410       3420
cctgaagcaa cggtttttgt tgacaactgc aatgtgatcc ttagggcaat aatggagaaa
      3430       3440       3450       3460       3470       3480
tgtggagatg tcgatgattg tgtctctact gtggaggcag ctgaacttgc agctaaactt
      3490       3500       3510       3520       3530       3540
gatgagaacc aaaagagtac cctgccactt cctggtcaag tagatttcat caacggaggg
      3550       3560       3570       3580       3590       3600
cctccatgcc aggggttttc tggtatgaat aggttcagtc acggctcttg gagtaaagtc
      3610       3620       3630       3640       3650       3660
cagtgtgaaa tgatattagc attcttgtct tttgctgatt atttccggcc aaagtacttt
      3670       3680       3690       3700       3710       3720
cttctcgaga acgtgaagaa atttgtgaca tacaataaag ggagaacatt tcaacttact
      3730       3740       3750       3760       3770       3780
atggcttctc ttcttgagat gggttaccag gtaagatttg gaatcttgga ggcaggtaca
      3790       3800       3810       3820       3830       3840
tatggagttt cccaacctcg taaaagagtt ataatttggg cagcttcacc agaagaagtt
      3850       3860       3870       3880       3890       3900
cttccagaat ggcctgagcc gatgcatgtc tttgataatc ccggtagtaa aatctcatta
      3910       3920       3930       3940       3950       3960
cctcgaggtt tacgctatga tgctggttgt aatactaaat ttggtgcacc ttttcgttca
      3970       3980       3990       4000       4010       4020
atcacggtga gagacacaat cggcgatctt ccaccagtag aaaacggaga atccaagata
      4030       4040       4050       4060       4070       4080
aacaaagagt atggaactac tccagcctcg tggttccaaa agaagataag aggaaacatg
      4090       4100       4110       4120       4130       4140
agtgttctca ctgatcatat ctgcaaaggg ctgaacgaac taaacctcat tcgatgtaag
      4150       4160       4170       4180       4190       4200
aaaatcccaa agaggcctgg tgctgattgg cgtgacctgc cggacgaaaa tgtgacatta
      4210       4220       4230       4240       4250       4260
tcaaatggac tcgtggaaaa attgcgtcct ttagctctat caaagacagc taaaaaccac
      4270       4280       4290       4300       4310       4320
aacgaatgga agggactcta tggtagattg gactggcaag gaaacttacc catttccatc
      4330       4340       4350       4360       4370       4380
accgatcctc agcccatggg taaggtggga atgtgcttcc atcctgaaca ggacagaata
      4390       4400       4410       4420       4430       4440
atcactgtcc gtgaatgcgc ccgatctcag gggtttccgg atagctacga gttttcaggg
      4450       4460       4470       4480       4490       4500
acgacaaaac acaaacatag gcaaattgga aatgcagtcc ctccaccatt ggccttcgct
      4510       4520       4530       4540       4550       4560
ctcggtcgga agctcaaaga agccctatat ctcaagagtt ctcttcaaca ccaatcataa

Last modification date: Oct. 2, 2011