Arabidopsis thaliana (L.) Heynh. (mouse-ear cress) [ATH]

FULL NAME: DNA (cytosine-5)-methyltransferase CMT3


DESCRIPTION:
Involved in the CpXpG methylation and in gene silencing. Methylates preferentially transposon-related sequences. Functionally redundant to DRM1/DRM2 to maintain non-CpG methylation. Involved in RNA-directed DNA methylation.

STRUCTURE SIMILARITY:
Belongs to the C5-methyltransferase family.
Contains 1 chromo domain.
Contains 1 BAH domain.


CATALYTIC ACTIVITY:
S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.


RELATED PATHWAY(S):
DNA replication
heterochromatin formation


Amino acids sequence

        10         20         30         40         50         60
MAPKRKRPAT KDDTTKSIPK PKKRAPKRAK TVKEEPVTVV EEGEKHVARF LDEPIPESEA
        70         80         90        100        110        120
KSTWPDRYKP IEVQPPKASS RKKTKDDEKV EIIRARCHYR RAIVDERQIY ELNDDAYVQS
       130        140        150        160        170        180
GEGKDPFICK IIEMFEGANG KLYFTARWFY RPSDTVMKEF EILIKKKRVF FSEIQDTNEL
       190        200        210        220        230        240
GLLEKKLNIL MIPLNENTKE TIPATENCDF FCDMNYFLPY DTFEAIQQET MMAISESSTI
       250        260        270        280        290        300
SSDTDIREGA AAISEIGECS QETEGHKKAT LLDLYSGCGA MSTGLCMGAQ LSGLNLVTKW
       310        320        330        340        350        360
AVDMNAHACK SLQHNHPETN VRNMTAEDFL FLLKEWEKLC IHFSLRNSPN SEEYANLHGL
       370        380        390        400        410        420
NNVEDNEDVS EESENEDDGE VFTVDKIVGI SFGVPKKLLK RGLYLKVRWL NYDDSHDTWE
       430        440        450        460        470        480
PIEGLSNCRG KIEEFVKLGY KSGILPLPGG VDVVCGGPPC QGISGHNRFR NLLDPLEDQK
       490        500        510        520        530        540
NKQLLVYMNI VEYLKPKFVL MENVVDMLKM AKGYLARFAV GRLLQMNYQV RNGMMAAGAY
       550        560        570        580        590        600
GLAQFRLRFF LWGALPSEII PQFPLPTHDL VHRGNIVKEF QGNIVAYDEG HTVKLADKLL
       610        620        630        640        650        660
LKDVISDLPA VANSEKRDEI TYDKDPTTPF QKFIRLRKDE ASGSQSKSKS KKHVLYDHHP
       670        680        690        700        710        720
LNLNINDYER VCQVPKRKGA NFRDFPGVIV GPGNVVKLEE GKERVKLESG KTLVPDYALT
       730        740        750        760        770        780
YVDGKSCKPF GRLWWDEIVP TVVTRAEPHN QVIIHPEQNR VLSIRENARL QGFPDDYKLF
       790        800        810        820        830
GPPKQKYIQV GNAVAVPVAK ALGYALGTAF QGLAVGKDPL LTLPEGFAFM KPTLPSELA

Encoded by CMT3 gene


OTHER NAME(S):
chromomethylase 3
CMT3
T6C23.3
T6C23_3


DESCRIPTION:
Encodes a chromomethylase involved in methylating cytosine residues at non-CG sites. Involved in preferentially methylating transposon-related sequences, reducing their mobility. CMT3 interacts with an Arabidopsis homologue of HP1 (heterochromatin protein 1), which in turn interacts with methylated histones. Involved in gene silencing.


Nucleic acid sequence

        10         20         30         40         50         60
atggcgccga agcgaaagag acctgcgaca aaggatgaca ctaccaaatc cattcccaaa
        70         80         90        100        110        120
ccgaagaaga gagctcctaa gcgagctaag acggtgaaag aagagccggt gacagtggtc
       130        140        150        160        170        180
gaggaagggg aaaagcatgt tgcgaggttt ctagacgagc caattccaga atctgaagcg
       190        200        210        220        230        240
aagagtacct ggcctgacag atacaaaccg attgaggtac agccacctaa ggcttcgtca
       250        260        270        280        290        300
agaaagaaga cgaaggatga cgaaaaagtt gagatcattc gtgctcgatg ccattataga
       310        320        330        340        350        360
cgtgcgattg ttgatgagcg tcagatatat gagctgaatg atgatgctta tgtacagtct
       370        380        390        400        410        420
ggtgagggaa aggatccctt catttgtaaa atcattgaaa tgtttgaagg ggctaatggg
       430        440        450        460        470        480
aaactgtatt tcacggctcg gtggttttat agaccttctg atactgtaat gaaagagttc
       490        500        510        520        530        540
gagattctga tcaagaaaaa gcgtgtgttt ttctctgaga tacaagatac aaatgaattg
       550        560        570        580        590        600
ggattacttg aaaagaagct gaacattttg atgattccct tgaatgaaaa tactaaagag
       610        620        630        640        650        660
actatccctg caacagaaaa ctgtgacttt ttctgtgaca tgaactattt cttgccttac
       670        680        690        700        710        720
gatacatttg aagctataca acaagaaacc atgatggcta taagtgaaag ttcaacaata
       730        740        750        760        770        780
tccagtgata ctgatataag agaaggagct gctgccatat cagagattgg agaatgttct
       790        800        810        820        830        840
caagaaacag aaggtcacaa aaaggcaact ttgcttgacc tttactccgg ctgtggagct
       850        860        870        880        890        900
atgtcgacag ggttgtgcat gggtgcacaa ctgtctggtt tgaacctcgt cactaaatgg
       910        920        930        940        950        960
gctgttgaca tgaatgcaca tgcatgtaaa agcttgcagc ataaccaccc agagacaaac
       970        980        990       1000       1010       1020
gtgagaaaca tgaccgcaga agatttcttg tttctgctta aggagtggga gaagctatgc
      1030       1040       1050       1060       1070       1080
attcatttct ctttgagaaa tagtccaaat tcagaagaat atgccaacct tcacggtttg
      1090       1100       1110       1120       1130       1140
aataatgttg aggacaatga agatgtcagc gaggagagtg aaaatgaaga tgatggagaa
      1150       1160       1170       1180       1190       1200
gtttttactg ttgacaagat tgttggtatt tccttcggag tccctaaaaa gttattgaaa
      1210       1220       1230       1240       1250       1260
cgtggacttt atttgaaggt aaggtggctg aattatgatg attctcatga tacatgggag
      1270       1280       1290       1300       1310       1320
cctattgaag gactcagtaa ttgccggggt aaaattgaag agttcgttaa acttggatat
      1330       1340       1350       1360       1370       1380
aaatctggca tccttccgtt accaggaggt gttgatgttg tctgcggtgg gccaccatgc
      1390       1400       1410       1420       1430       1440
caaggaatca gtggtcacaa ccgcttcagg aacttattgg accctctaga agatcagaaa
      1450       1460       1470       1480       1490       1500
aacaagcagc ttttggtgta tatgaacatt gtagaatatt tgaagcctaa gttcgttttg
      1510       1520       1530       1540       1550       1560
atggaaaacg tcgttgacat gctgaagatg gctaagggct atcttgcacg gtttgctgtt
      1570       1580       1590       1600       1610       1620
ggacgccttc tacagatgaa ttaccaagtg aggaatggaa tgatggcagc tggagcttat
      1630       1640       1650       1660       1670       1680
gggcttgctc agtttcgttt gaggttcttt ctatggggtg cactccctag tgagataatt
      1690       1700       1710       1720       1730       1740
ccgcagttcc cacttccaac acatgatcta gttcatagag gaaatattgt caaggagttt
      1750       1760       1770       1780       1790       1800
cagggaaaca tagtagccta tgatgaagga catactgtga agttagcaga caagcttttg
      1810       1820       1830       1840       1850       1860
ttgaaggatg tgatttctga tcttcctgca gttgccaaca gtgaaaaaag agacgagatt
      1870       1880       1890       1900       1910       1920
acatatgaca aagatcccac aacgccattt caaaagttca tcagattgag aaaggatgaa
      1930       1940       1950       1960       1970       1980
gcgtcaggtt cacaatcaaa gtccaagtcc aaaaagcatg tcttatatga tcatcaccct
      1990       2000       2010       2020       2030       2040
cttaatctta atataaatga ctatgaacgg gtttgtcagg tccccaagag aaagggagcg
      2050       2060       2070       2080       2090       2100
aattttaggg actttcctgg tgttattgtt ggacctggta atgtagtcaa gttggaagag
      2110       2120       2130       2140       2150       2160
ggaaaggaaa gggtcaaact tgaatctgga aaaacattgg ttcccgatta tgccttaaca
      2170       2180       2190       2200       2210       2220
tatgtcgatg ggaaatcatg caaacctttt ggtcgtcttt ggtgggacga aattgtcccc
      2230       2240       2250       2260       2270       2280
actgttgtca cacgggcaga accccacaac caggtgatca ttcatccaga gcaaaatcgg
      2290       2300       2310       2320       2330       2340
gttttatcca ttcgagaaaa tgcgagactc caaggctttc ctgatgacta caaactcttt
      2350       2360       2370       2380       2390       2400
ggcccaccca aacagaagta cattcaagta ggtaacgctg tagctgtgcc agtagcgaag
      2410       2420       2430       2440       2450       2460
gcccttggat atgctttggg aacagctttc cagggactcg cagttgggaa agatccactt
      2470       2480       2490       2500       2510       2520
cttactctgc ctgaaggttt tgcattcatg aagccaactc ttccttccga gcttgcatga

Last modification date: Oct. 2, 2011