Drosophila melanogaster Meigen (fruit fly) [DME]

FULL NAME: Ribonuclease H2 subunit A


DESCRIPTION:
Catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes (By similarity).

STRUCTURE SIMILARITY:
Belongs to the RNase HII family.


CATALYTIC ACTIVITY:
Endonucleolytic cleavage to 5'-phosphomonoester. Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding By similarity.


PROTEIN TYPE(S):
RNase


RELATED PATHWAY(S):
DNA replication


Amino acids sequence

        10         20         30         40         50         60
MSDQEDVVVK KEKKCIENID ENDEEPDDVD FKDSGKISSL KALGPFIAGK ENSRNTVYLS
        70         80         90        100        110        120
DVPDICKDKP CMLGVDEAGR GPVLGPMVYG ISYCPLESNK ALEDLGCADS KQLTEGKRDI
       130        140        150        160        170        180
IFNDINTKEY ATSCVGWAVE IISPNTISTS MYRRSKCSLN EVSMDSAMGL IQQAIDAGVN
       190        200        210        220        230        240
IAEVYVDTVG PPEKYQEKLL KRFPSFKITV AKKADSTYPI VSAASICAKV TRDHALKVWS
       250        260        270        280        290        300
FPEGLVIKDN EFGSGYPGDP VTRRFLTEYI DLVFGFPRLV RFSWSTAENA LADKAYDMEF
       310        320        330        340
DEPDSEKPKY AGTKLTKFFK GTTKSGEIIR EECRFFKQRH LESVMEF 

Encoded by CG13690 gene


OTHER NAME(S):
Dmel\CG13690


Nucleic acid sequence

        10         20         30         40         50         60
atgtcggacc aagaagacgt agttgtaaaa aaagaaaaaa aatgtataga aaatattgat
        70         80         90        100        110        120
gagaacgacg aagagcccga tgatgtagat tttaaggaca gcggaaaaat aagctccttg
       130        140        150        160        170        180
aaggccctgg gtccatttat agccggcaag gagaatagcc gcaacacggt ttacctcagc
       190        200        210        220        230        240
gatgttccgg atatttgcaa ggataagccg tgcatgctgg gcgtggacga agcagggcgt
       250        260        270        280        290        300
ggtcccgtcc tgggacctat ggtatatggg atctcctact gtccactgga aagtaacaag
       310        320        330        340        350        360
gctttagagg acctgggatg cgccgattcc aaacaactaa ccgaggggaa acgtgatatc
       370        380        390        400        410        420
atatttaacg atatcaacac caaggaatat gcgaccagtt gcgttggctg ggccgtggag
       430        440        450        460        470        480
atcatatcgc cgaacacgat cagcaccagc atgtaccggc ggtcgaagtg ctccctgaac
       490        500        510        520        530        540
gaagtttcca tggactcggc catgggcctt atccaacagg cgatcgatgc gggcgtcaat
       550        560        570        580        590        600
atagccgagg tttatgtgga tacggtgggt ccgccggaga agtaccagga gaagttgctc
       610        620        630        640        650        660
aaacgtttcc ccagctttaa aatcaccgtg gcgaaaaaag ctgactccac ctatccaata
       670        680        690        700        710        720
gtctctgctg ctagtatatg cgccaaagtt acccgtgatc atgccctcaa ggtgtggagc
       730        740        750        760        770        780
ttccccgagg gcttggtcat taaggacaac gagttcggca gtggatatcc gggagatccg
       790        800        810        820        830        840
gtcacaagaa gatttctgac cgagtacatc gatctagtgt ttggatttcc acgacttgtg
       850        860        870        880        890        900
cgcttcagtt ggtccaccgc ggaaaacgca ctggcggaca aggcctatga catggagttc
       910        920        930        940        950        960
gacgagccgg attccgagaa gcccaagtac gccggcacca agctcacaaa gttctttaag
       970        980        990       1000       1010       1020
ggcacaacaa aatccggcga aataattcgc gaggagtgcc gcttctttaa gcaacgccat
      1030       1040
ctggaaagtg tcatggagtt ttag   

Last modification date: Nov. 9, 2011