Homo sapiens L. (human) [HSA]

FULL NAME: DNA replication licensing factor MCM6


DESCRIPTION:
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.

MISCELLANEOUS:
Early fractionation of eukaryotic MCM proteins yielded a variety of dimeric, trimeric and tetrameric complexes with unclear biological significance. Specifically a MCM467 subcomplex is shown to have in vitro helicase activity which is inhibited by the MCM2 subunit. The MCM2-7 hexamer is the proposed physiological active complex.


STRUCTURE SIMILARITY:
Contains 1 MCM domain.
Belongs to the MCM family.


CATALYTIC ACTIVITY:
ATP + H(2)O = ADP + phosphate.


POST-TRANSLATIONAL MODIFICATION:
Phosphorylated upon DNA damage, probably by ATM or ATR.


RELATED PATHWAY(S):
DNA replication


Amino acids sequence

        10         20         30         40         50         60
MDLAAAAEPG AGSQHLEVRD EVAEKCQKLF LDFLEEFQSS DGEIKYLQLA EELIRPERNT
        70         80         90        100        110        120
LVVSFVDLEQ FNQQLSTTIQ EEFYRVYPYL CRALKTFVKD RKEIPLAKDF YVAFQDLPTR
       130        140        150        160        170        180
HKIRELTSSR IGLLTRISGQ VVRTHPVHPE LVSGTFLCLD CQTVIRDVEQ QFKYTQPNIC
       190        200        210        220        230        240
RNPVCANRRR FLLDTNKSRF VDFQKVRIQE TQAELPRGSI PRSLEVILRA EAVESAQAGD
       250        260        270        280        290        300
KCDFTGTLIV VPDVSKLSTP GARAETNSRV SGVDGYETEG IRGLRALGVR DLSYRLVFLA
       310        320        330        340        350        360
CCVAPTNPRF GGKELRDEEQ TAESIKNQMT VKEWEKVFEM SQDKNLYHNL CTSLFPTIHG
       370        380        390        400        410        420
NDEVKRGVLL MLFGGVPKTT GEGTSLRGDI NVCIVGDPST AKSQFLKHVE EFSPRAVYTS
       430        440        450        460        470        480
GKASSAAGLT AAVVRDEESH EFVIEAGALM LADNGVCCID EFDKMDVRDQ VAIHEAMEQQ
       490        500        510        520        530        540
TISITKAGVK ATLNARTSIL AAANPISGHY DRSKSLKQNI NLSAPIMSRF DLFFILVDEC
       550        560        570        580        590        600
NEVTDYAIAR RIVDLHSRIE ESIDRVYSLD DIRRYLLFAR QFKPKISKES EDFIVEQYKH
       610        620        630        640        650        660
LRQRDGSGVT KSSWRITVRQ LESMIRLSEA MARMHCCDEV QPKHVKEAFR LLNKSIIRVE
       670        680        690        700        710        720
TPDVNLDQEE EIQMEVDEGA GGINGHADSP APVNGINGYN EDINQESAPK ASLRLGFSEY
       730        740        750        760        770        780
CRISNLIVLH LRKVEEEEDE SALKRSELVN WYLKEIESEI DSEEELINKK RIIEKVIHRL
       790        800        810        820
THYDHVLIEL TQAGLKGSTE GSESYEEDPY LVVNPNYLLE D 

Encoded by MCM6 gene

FULL NAME: minichromosome maintenance complex component 6


OTHER NAME(S):
MCG40308
Mis5
P105MCM


DESCRIPTION:
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication. [provided by RefSeq, Jul 2008]


Nucleic acid sequence

        10         20         30         40         50         60
atggacctcg cggcggcagc ggagccgggc gccggcagcc agcacctgga ggtccgcgac
        70         80         90        100        110        120
gaggtggccg agaagtgcca gaaactgttc ctggacttct tggaggagtt tcagagcagc
       130        140        150        160        170        180
gatggagaaa ttaaatactt gcaattagca gaggaactga ttcgtcctga gagaaacaca
       190        200        210        220        230        240
ttggttgtga gttttgtgga cctggaacaa tttaaccagc aactttccac caccattcaa
       250        260        270        280        290        300
gaggagttct atagagttta cccttacctg tgtcgggcct tgaaaacatt cgtcaaagac
       310        320        330        340        350        360
cgtaaagaga tccctcttgc caaggatttt tatgttgcat tccaagacct gcctaccaga
       370        380        390        400        410        420
cacaagattc gagagctcac ctcatccaga attggtttgc tcactcgcat cagtgggcag
       430        440        450        460        470        480
gtggtgcgga ctcacccagt tcacccagag cttgtgagcg gaacttttct gtgcttggac
       490        500        510        520        530        540
tgtcagacag tgatcaggga tgtagaacag cagttcaaat acacacagcc aaacatctgc
       550        560        570        580        590        600
cgaaatccag tttgtgccaa caggaggaga ttcttactgg atacaaataa atcaagattt
       610        620        630        640        650        660
gttgattttc aaaaggttcg tattcaagag acccaagctg agcttcctcg agggagtatc
       670        680        690        700        710        720
ccccgcagtt tagaagtaat tttaagggct gaagctgtgg aatcagctca agctggtgac
       730        740        750        760        770        780
aagtgtgact ttacagggac actgattgtt gtgcctgacg tctccaagct tagcacacca
       790        800        810        820        830        840
ggagcacgtg cagaaactaa ttcccgtgtc agtggtgttg atggatatga gacagaaggc
       850        860        870        880        890        900
attcgaggac tccgggccct tggtgttagg gacctttctt ataggctggt ctttcttgcc
       910        920        930        940        950        960
tgctgtgttg cgccaaccaa cccaaggttt ggggggaaag agctcagaga tgaggaacag
       970        980        990       1000       1010       1020
acagctgaga gcattaagaa ccaaatgact gtgaaagaat gggagaaagt gtttgagatg
      1030       1040       1050       1060       1070       1080
agtcaagata aaaatctata ccacaatctt tgtaccagcc tgttccctac tatacatggc
      1090       1100       1110       1120       1130       1140
aatgatgaag taaaacgggg tgtcctgctg atgctctttg gtggcgttcc aaagacaaca
      1150       1160       1170       1180       1190       1200
ggagaaggga cctctcttcg aggggacata aatgtttgca ttgttggtga cccaagtaca
      1210       1220       1230       1240       1250       1260
gctaagagcc aatttctcaa gcacgtggag gagttcagcc ccagagctgt ctacaccagt
      1270       1280       1290       1300       1310       1320
ggtaaagcgt ccagtgctgc tggcttaaca gcagctgttg tgagagatga agaatctcat
      1330       1340       1350       1360       1370       1380
gagtttgtca ttgaggctgg agctttgatg ttggctgata atggtgtgtg ttgtattgat
      1390       1400       1410       1420       1430       1440
gaatttgata agatggacgt gcgggatcaa gttgctattc atgaagctat ggaacagcag
      1450       1460       1470       1480       1490       1500
accatatcca tcactaaagc aggagtgaag gctactctga acgcccggac gtccattttg
      1510       1520       1530       1540       1550       1560
gcagcagcaa acccaatcag tggacactat gacagatcaa aatcattgaa acagaatata
      1570       1580       1590       1600       1610       1620
aatttgtcag ctcccatcat gtcccgattc gatctcttct ttatccttgt ggatgaatgt
      1630       1640       1650       1660       1670       1680
aatgaggtta cagattatgc cattgccagg cgcatagtag atttgcattc aagaattgag
      1690       1700       1710       1720       1730       1740
gaatcaattg atcgtgtcta ttccctcgat gatatcagaa gatatcttct ctttgcaaga
      1750       1760       1770       1780       1790       1800
cagtttaaac ccaagatttc caaagagtca gaggacttca ttgtggagca atataaacat
      1810       1820       1830       1840       1850       1860
ctccgccaga gagatggttc tggagtgacc aagtcttcat ggaggattac agtgcgacag
      1870       1880       1890       1900       1910       1920
cttgagagca tgattcgtct ctctgaagct atggctcgga tgcactgctg tgatgaggtc
      1930       1940       1950       1960       1970       1980
caacctaaac atgtgaagga agctttccgg ttactgaata aatcaatcat ccgtgtggaa
      1990       2000       2010       2020       2030       2040
acacctgatg tcaatctaga tcaagaggaa gagatccaga tggaggtaga tgagggtgct
      2050       2060       2070       2080       2090       2100
ggtggcatca atggtcatgc tgacagccct gctcctgtga acgggatcaa tggctacaat
      2110       2120       2130       2140       2150       2160
gaagacataa atcaagagtc tgctcccaaa gcctccttaa ggctgggctt ctctgagtac
      2170       2180       2190       2200       2210       2220
tgccgaatct ctaaccttat tgtgcttcac ctcagaaagg tggaagaaga agaggacgag
      2230       2240       2250       2260       2270       2280
tcagcattaa agaggagcga gcttgttaac tggtacttga aggaaatcga atcagagata
      2290       2300       2310       2320       2330       2340
gactctgaag aagaacttat aaataaaaaa agaatcatag agaaagttat tcatcgactc
      2350       2360       2370       2380       2390       2400
acacactatg atcatgttct aattgagctc acccaggctg gattgaaagg ctccacagag
      2410       2420       2430       2440       2450       2460
ggaagtgaga gctatgaaga agatccctac ttggtagtta accctaacta cttgctcgaa

gattga     

Last modification date: Oct. 2, 2011