Mus musculus L. (mouse) [MMU]

FULL NAME: Proliferating cell nuclear antigen


DESCRIPTION:
This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities (By similarity). Has to be loaded onto DNA in order to be able to stimulate APEX2 (By similarity).

STRUCTURE SIMILARITY:
Belongs to the PCNA family.


POST-TRANSLATIONAL MODIFICATION:
Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical poly-ubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis (By similarity).
Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation (By similarity).


PROTEIN TYPE(S):
accessory protein


RELATED PATHWAY(S):
DNA replication
base excision repair (BER)
nucleotide excision repair (NER)
mismatch repair (MMR)


Amino acids sequence

        10         20         30         40         50         60
MFEARLIQGS ILKKVLEALK DLINEACWDV SSGGVNLQSM DSSHVSLVQL TLRSEGFDTY
        70         80         90        100        110        120
RCDRNLAMGV NLTSMSKILK CAGNEDIITL RAEDNADTLA LVFEAPNQEK VSDYEMKLMD
       130        140        150        160        170        180
LDVEQLGIPE QEYSCVIKMP SGEFARICRD LSHIGDAVVI SCAKNGVKFS ASGELGNGNI
       190        200        210        220        230        240
KLSQTSNVDK EEEAVTIEMN EPVHLTFALR YLNFFTKATP LSPTVTLSMS ADVPLVVEYK
       250        260
IADMGHLKYY LAPKIEDEEA S   

Encoded by Pcna gene

FULL NAME: proliferating cell nuclear antigen


Nucleic acid sequence

        10         20         30         40         50         60
atgtttgagg cacgcctgat ccagggctcc atcctgaaga aggtgctgga ggctctcaaa
        70         80         90        100        110        120
gacctcatca atgaggcctg ctgggacgtc agctcgggcg gcgtgaacct gcagagcatg
       130        140        150        160        170        180
gactcgtctc acgtctcctt ggtacagctt actctgcgct ccgaaggctt cgacacatac
       190        200        210        220        230        240
cgctgcgacc gcaacctagc catgggcgtg aacctcacca gcatgtccaa aattctaaaa
       250        260        270        280        290        300
tgtgctggta atgaagacat cattacatta agggctgaag ataatgcaga caccttagca
       310        320        330        340        350        360
ctagtattcg aagcaccaaa tcaagagaaa gtttcagact atgaaatgaa gttaatggac
       370        380        390        400        410        420
ttagatgtgg agcaacttgg aatcccagaa caggagtaca gctgtgtaat aaagatgccg
       430        440        450        460        470        480
tcgggtgaat ttgcacgtat atgccgagac cttagccaca ttggagatgc tgttgtgata
       490        500        510        520        530        540
tcctgtgcaa agaatggggt gaagttttct gcaagtggag agcttggcaa tgggaacatt
       550        560        570        580        590        600
aagttgtcac aaacaagtaa tgtggataaa gaagaggagg cggtaaccat agagatgaat
       610        620        630        640        650        660
gagcctgttc acctaacgtt tgctctgagg tacctgaact ttttcacaaa agccactcca
       670        680        690        700        710        720
ctgtctccta cagtaacact cagtatgtct gcagatgtgc cccttgttgt agagtataaa
       730        740        750        760        770        780
attgctgaca tgggacactt aaagtattat ttggctccca agattgaaga tgaggaagca

tcttag     

Last modification date: Oct. 2, 2011