Homo sapiens L. (human) [HSA]

FULL NAME: DNA replication licensing factor MCM3


DESCRIPTION:
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.

MISCELLANEOUS:
Early fractionation of eukaryotic MCM proteins yielded a variety of dimeric, trimeric and tetrameric complexes with unclear biological significance. The MCM2-7 hexamer is the proposed physiological active complex.


STRUCTURE SIMILARITY:
Contains 1 MCM domain.
Belongs to the MCM family.


CATALYTIC ACTIVITY:
ATP + H(2)O = ADP + phosphate.


POST-TRANSLATIONAL MODIFICATION:
Phosphorylated upon DNA damage, probably by ATM or ATR.


RELATED PATHWAY(S):
DNA replication


Amino acids sequence

        10         20         30         40         50         60
MAGTVVLDDV ELREAQRDYL DFLDDEEDQG IYQSKVRELI SDNQYRLIVN VNDLRRKNEK
        70         80         90        100        110        120
RANRLLNNAF EELVAFQRAL KDFVASIDAT YAKQYEEFYV GLEGSFGSKH VSPRTLTSCF
       130        140        150        160        170        180
LSCVVCVEGI VTKCSLVRPK VVRSVHYCPA TKKTIERRYS DLTTLVAFPS SSVYPTKDEE
       190        200        210        220        230        240
NNPLETEYGL SVYKDHQTIT IQEMPEKAPA GQLPRSVDVI LDDDLVDKAK PGDRVQVVGT
       250        260        270        280        290        300
YRCLPGKKGG YTSGTFRTVL IACNVKQMSK DAQPSFSAED IAKIKKFSKT RSKDIFDQLA
       310        320        330        340        350        360
KSLAPSIHGH DYVKKAILCL LLGGVERDLE NGSHIRGDIN ILLIGDPSVA KSQLLRYVLC
       370        380        390        400        410        420
TAPRAIPTTG RGSSGVGLTA AVTTDQETGE RRLEAGAMVL ADRGVVCIDE FDKMSDMDRT
       430        440        450        460        470        480
AIHEVMEQGR VTIAKAGIHA RLNARCSVLA AANPVYGRYD QYKTPMENIG LQDSLLSRFD
       490        500        510        520        530        540
LLFIMLDQMD PEQDREISDH VLRMHRYRAP GEQDGDAMPL GSAVDILATD DPNFSQEDQQ
       550        560        570        580        590        600
DTQIYEKHDN LLHGTKKKKE KMVSAAFMKK YIHVAKIIKP VLTQESATYI AEEYSRLRSQ
       610        620        630        640        650        660
DSMSSDTART SPVTARTLET LIRLATAHAK ARMSKTVDLQ DAEEAVELVQ YAYFKKVLEK
       670        680        690        700        710        720
EKKRKKRSED ESETEDEEEK SQEDQEQKRK RRKTRQPDAK DGDSYDPYDF SDTEEEMPQV
       730        740        750        760        770        780
HTPKTADSQE TKESQKVELS ESRLKAFKVA LLDVFREAHA QSIGMNRLTE SINRDSEEPF
       790        800
SSVEIQAALS KMQDDNQVMV SEGIIFLI   

Encoded by MCM3 gene

FULL NAME: minichromosome maintenance complex component 3


OTHER NAME(S):
HCC5
MGC1157
P1-MCM3
P1.h
RLFB


DESCRIPTION:
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. [provided by RefSeq, Jul 2008]


Nucleic acid sequence

        10         20         30         40         50         60
atggcgggta ccgtggtgct ggacgatgtg gagctgcggg aggctcagag agattacctg
        70         80         90        100        110        120
gacttcctgg acgacgagga agaccaggga atttatcaga gcaaagttcg ggagctgatc
       130        140        150        160        170        180
agtgacaacc aataccggct gattgtcaat gtgaatgacc tgcgcaggaa aaacgagaag
       190        200        210        220        230        240
agggctaacc ggcttctgaa caatgccttt gaggagctgg ttgccttcca gcgggcctta
       250        260        270        280        290        300
aaggattttg tggcctccat tgatgctacc tatgccaagc agtatgagga gttctacgta
       310        320        330        340        350        360
ggactggaag gcagctttgg ctccaagcac gtctccccgc ggactcttac ctcctgcttc
       370        380        390        400        410        420
ctcagctgtg tggtctgtgt ggagggcatt gtcactaaat gttctctagt tcgtcccaaa
       430        440        450        460        470        480
gtcgtccgca gtgtccacta ctgtcctgct actaagaaga ccatagagcg acgttattct
       490        500        510        520        530        540
gatctcacca ccctggtggc ctttccctcc agctctgtct atcctaccaa ggatgaggag
       550        560        570        580        590        600
aacaatcccc ttgagacaga atatggcctt tctgtctaca aggatcacca gaccatcacc
       610        620        630        640        650        660
atccaggaga tgccggagaa ggccccagcc ggccagctcc cccgctctgt ggacgtcatt
       670        680        690        700        710        720
ctggatgatg acttggtgga taaagcgaag cctggtgacc gggttcaggt ggtgggaacc
       730        740        750        760        770        780
taccgttgcc ttcctggaaa gaagggaggc tacacctctg ggaccttcag gactgtcctg
       790        800        810        820        830        840
attgcctgta atgttaagca gatgagcaag gatgctcagc cctctttctc tgctgaggat
       850        860        870        880        890        900
atagccaaga tcaagaagtt cagtaaaacc cgatccaagg atatctttga ccagctggcc
       910        920        930        940        950        960
aagtcattgg ccccaagtat ccatgggcat gactatgtca agaaagcaat cctctgcttg
       970        980        990       1000       1010       1020
ctcttgggag gggtggaacg agacctagaa aatggcagcc acatccgtgg ggacatcaat
      1030       1040       1050       1060       1070       1080
attcttctaa taggagaccc atccgttgcc aagtctcagc ttctgcggta tgtgctttgc
      1090       1100       1110       1120       1130       1140
actgcacccc gagctatccc caccactggc cggggctcct ctggagtggg tctgacggct
      1150       1160       1170       1180       1190       1200
gctgtcacca cagaccagga aacaggagag cgccgtctgg aagcaggggc catggtcctg
      1210       1220       1230       1240       1250       1260
gctgaccgag gcgtggtttg cattgatgaa tttgacaaaa tgtctgacat ggatcgcaca
      1270       1280       1290       1300       1310       1320
gccatccatg aagtgatgga gcagggtcga gtgaccattg ccaaggctgg catccatgct
      1330       1340       1350       1360       1370       1380
cggctgaatg cccgctgcag tgttttggca gctgccaacc ctgtctacgg caggtatgac
      1390       1400       1410       1420       1430       1440
cagtataaga ctccaatgga gaacattggg ctacaggact cactgctgtc acgatttgac
      1450       1460       1470       1480       1490       1500
ttgctcttca tcatgctgga tcagatggat cctgagcagg atcgggagat ctcagaccat
      1510       1520       1530       1540       1550       1560
gtccttcgga tgcaccgtta cagagcacct ggggagcagg atggcgatgc tatgcccttg
      1570       1580       1590       1600       1610       1620
ggtagtgctg tggatatcct ggccacagat gatcccaact ttagccagga agatcagcag
      1630       1640       1650       1660       1670       1680
gacacccaga tttatgagaa gcatgacaac cttctacatg ggaccaagaa gaaaaaggag
      1690       1700       1710       1720       1730       1740
aagatggtga gtgcagcatt catgaagaag tacatccatg tggccaaaat catcaagcct
      1750       1760       1770       1780       1790       1800
gtcctgacac aggagtcggc cacctacatt gcagaagagt attcacgcct gcgcagccag
      1810       1820       1830       1840       1850       1860
gatagcatga gctcagacac cgccaggaca tctccagtta cagcccgaac actggaaact
      1870       1880       1890       1900       1910       1920
ctgattcgac tggccacagc ccatgcgaag gcccgcatga gcaagactgt ggacctgcag
      1930       1940       1950       1960       1970       1980
gatgcagagg aagctgtgga gttggtccag tatgcttact ttaagaaggt tctggagaag
      1990       2000       2010       2020       2030       2040
gagaagaaac gtaagaagcg aagtgaggat gaatcagaga cagaagatga agaggagaaa
      2050       2060       2070       2080       2090       2100
agccaagagg accaggagca gaagaggaag agaaggaaga ctcgccagcc agatgccaaa
      2110       2120       2130       2140       2150       2160
gatggggatt catacgaccc ctatgacttc agtgacacag aggaggaaat gcctcaagta
      2170       2180       2190       2200       2210       2220
cacactccaa agacggcaga ctcacaggag accaaggaat cccagaaagt ggagttgagt
      2230       2240       2250       2260       2270       2280
gaatccaggt tgaaggcatt caaggtggcc ctcttggatg tgttccggga agctcatgcg
      2290       2300       2310       2320       2330       2340
cagtcaatcg gcatgaatcg cctcacagaa tccatcaacc gggacagcga agagcccttc
      2350       2360       2370       2380       2390       2400
tcttcagttg agatccaggc tgctctgagc aagatgcagg atgacaatca ggtcatggtg
      2410       2420
tctgagggca tcatcttcct catctga   

Last modification date: Oct. 2, 2011