Mus musculus L. (mouse) [MMU]

FULL NAME: DNA replication licensing factor MCM2


DESCRIPTION:
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.

STRUCTURE SIMILARITY:
Contains 1 MCM domain.
Belongs to the MCM family.


CATALYTIC ACTIVITY:
ATP + H(2)O = ADP + phosphate.


POST-TRANSLATIONAL MODIFICATION:
Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication (By similarity).


RELATED PATHWAY(S):
DNA replication


Amino acids sequence

        10         20         30         40         50         60
MAESSESLSA SSPARQRRRI SDPLTSSPGR SSRRADALTS SPGRDLPPFE DESEGLLGTE
        70         80         90        100        110        120
GPMEEEEDGE ELIGDGMERD YRPIPELDVY EAEGLALDDE DVEELTASQR EAAERTMRQR
       130        140        150        160        170        180
DREAGRGLGR MRRGLLYDSS EEDEERPARK RRHVERATED GEEDEEMIES IENLEDLKGH
       190        200        210        220        230        240
SVREWVSMAG PRLEIHHRFK NFLRTHVDSH GHNVFKERIS DMCKENRESL VVNYEDLAAR
       250        260        270        280        290        300
EHVLAYFLPE APAELLQIFD EAALEVVLAM YPKYDRITNH IHVRISHLPL VEELRSLRQL
       310        320        330        340        350        360
HLNQLIRTSG VVTSCTGVLP QLSMVKYNCS KCNFVLGPFC QSQNQEVKPG SCPECQSAGP
       370        380        390        400        410        420
FEINMEETIY QNYQRIRIQE SPGKVAAGRL PRSKDAILLA DLVDSCKPGD EIELTGIYHN
       430        440        450        460        470        480
NYDGSLNTAN GFPVFATIIL ANHVAKKDNK VAVGELTDED VKMITGLSKD QQIGEKIFAS
       490        500        510        520        530        540
IAPSIYGHED IKRGLALALF GGEPKNPGGK HKVRGDINVL LCGDPGTAKS QFLKYIEKVS
       550        560        570        580        590        600
SRAIFTTGQG ASAVGLTAYV QRHPVSREWT LEAGALVLAD RGVCLIDEFD KMNDQDRTSI
       610        620        630        640        650        660
HEAMEQQSIS ISKAGIVTSL QARCTVIAAA NPIGGRYDPS LTFSENVDLT EPIISRFDVL
       670        680        690        700        710        720
CVVRDTVDPV QDEMLARFVV GSHVRHHPSN KKDEGLTNGG TLEPAMPNTY GVEPLPQEVL
       730        740        750        760        770        780
KKYIIYAKER VRPKLNQMDQ DKVARMYSDL RKESMATGSI PITVRHIESM IRMAEAHARM
       790        800        810        820        830        840
HLRDYVMEDD VNMAIRVMME SFIDTQKFSV MRSMRKTFAR YLSFRRDNND LLLFILKQLV
       850        860        870        880        890        900
AEQVTYQRNR FGAQQDTIEI PEKDLMDKAR QINIHNLSAF YDSDLFKFNK FSRDLKRKLI

LQQF     

Encoded by Mcm2 gene

FULL NAME: minichromosome maintenance deficient 2 mitotin (S. cerevisiae)


OTHER NAME(S):
AA959861
AW476101
BM28
CDCL1
Mcmd2
mKIAA0030


Nucleic acid sequence

        10         20         30         40         50         60
atggcggagt cttctgagtc tctctcagca tctagccctg cccgtcagcg gcgccggatc
        70         80         90        100        110        120
agtgatcccc tcacctccag cccaggccgc agctccagac gtgctgacgc cctgacctcc
       130        140        150        160        170        180
agccctggca gagacctccc cccatttgaa gatgagtctg aggggctgct gggcacagag
       190        200        210        220        230        240
gggcccatgg aggaagaaga ggatggagag gaactcattg gtgatggcat ggagagagac
       250        260        270        280        290        300
taccgtccca ttccggagct cgatgtctac gaggccgagg gattggccct ggatgatgaa
       310        320        330        340        350        360
gatgtggagg agctgacagc cagtcagaga gaggcagctg agcggaccat gaggcagcgg
       370        380        390        400        410        420
gaccgtgagg ctggcagagg cctgggacgc atgcgccggg ggctgctcta tgacagcagc
       430        440        450        460        470        480
gaggaagatg aggagcggcc tgcccgtaag cgccgccacg tagaacgcgc cacagaggat
       490        500        510        520        530        540
ggcgaggagg atgaagagat gatcgagagt attgagaatc tggaggacct caagggccac
       550        560        570        580        590        600
tcggtgcgcg agtgggtgag catggcaggg cccaggctgg agatccacca ccgcttcaag
       610        620        630        640        650        660
aacttcctgc gcacccacgt ggacagccat ggccacaacg tcttcaagga gcgcatcagt
       670        680        690        700        710        720
gatatgtgca aagagaaccg tgagagtttg gtggtaaatt atgaagacct ggcagcccgg
       730        740        750        760        770        780
gagcacgtgt tggcatactt cctgccggaa gcaccggctg agttgctgca gatctttgac
       790        800        810        820        830        840
gaggctgccc tggaggtcgt gttggccatg taccctaaat atgaccgtat caccaaccac
       850        860        870        880        890        900
atccatgtgc gcatctccca cctgcctctg gtggaggagc tgcgttcact gaggcagttg
       910        920        930        940        950        960
cacctgaacc agctgatccg taccagtggc gtggtgacca gctgcaccgg agtcctgccc
       970        980        990       1000       1010       1020
cagctcagca tggtcaagta caactgtagc aagtgcaact ttgtactggg gcctttctgc
      1030       1040       1050       1060       1070       1080
cagtctcaga atcaggaggt gaagcctggc tcctgccctg agtgccagtc tgctgggccc
      1090       1100       1110       1120       1130       1140
tttgagatca acatggagga gaccatctat cagaactacc aacgtatccg catccaggag
      1150       1160       1170       1180       1190       1200
agtcccggca aggtggcggc tggccgactg ccccgttcca aggatgccat tctcctcgct
      1210       1220       1230       1240       1250       1260
gatctggtgg acagctgcaa gccaggggac gagatagagc tgaccggcat ttaccataat
      1270       1280       1290       1300       1310       1320
aactatgacg gctcgcttaa caccgccaac ggctttccag tctttgccac tattatcttg
      1330       1340       1350       1360       1370       1380
gccaaccatg ttgccaagaa ggacaacaaa gtagctgtgg gggagctcac cgatgaggac
      1390       1400       1410       1420       1430       1440
gtgaagatga tcaccggtct ctccaaggat cagcaaattg gagagaagat ctttgccagc
      1450       1460       1470       1480       1490       1500
attgcaccct ccatctatgg gcatgaagac atcaagagag gcctggctct ggccctgttt
      1510       1520       1530       1540       1550       1560
ggaggggagc ccaagaaccc aggtggaaag cacaaggttc gaggtgacat taatgtgctc
      1570       1580       1590       1600       1610       1620
ttgtgtgggg accctggcac agcaaagtcc caattcctca aatacatcga gaaagtgtct
      1630       1640       1650       1660       1670       1680
agccgtgcca tcttcaccac tggccagggt gcgtcagcag tgggtctcac cgcgtacgtt
      1690       1700       1710       1720       1730       1740
cagcggcatc ccgtcagcag agagtggacc ttagaggcgg gagccctggt tctggctgac
      1750       1760       1770       1780       1790       1800
cggggggtgt gtctcattga cgagtttgac aagatgaatg accaggacag gaccagcatc
      1810       1820       1830       1840       1850       1860
cacgaggcca tggaacagca aagcatctcc atctccaagg ctggcatcgt tacctcgctg
      1870       1880       1890       1900       1910       1920
caagcccgct gcactgtcat agctgctgcc aaccccatag gaggccgcta cgacccttca
      1930       1940       1950       1960       1970       1980
ctgaccttct cagagaatgt agacctcaca gagcccatca tttcccgctt tgatgtcctg
      1990       2000       2010       2020       2030       2040
tgtgtggtga gggacactgt tgatccagtt caggatgaga tgctggcccg ctttgtggtt
      2050       2060       2070       2080       2090       2100
ggcagccacg tcagacacca ccccagtaac aagaaggatg aagggttgac taatggtggc
      2110       2120       2130       2140       2150       2160
accttggagc cagccatgcc caacacatat ggcgtggagc ccctgcctca ggaggtgctg
      2170       2180       2190       2200       2210       2220
aagaagtata tcatctatgc caaggagagg gtccgcccga agctcaacca gatggaccag
      2230       2240       2250       2260       2270       2280
gataaagtgg ccaggatgta cagtgacctg aggaaggagt ccatggcaac gggcagcatt
      2290       2300       2310       2320       2330       2340
cccatcacgg tgcgccacat cgagtccatg atccgcatgg ccgaggccca tgcccgcatg
      2350       2360       2370       2380       2390       2400
cacctgcggg actacgtgat ggaagacgat gtcaacatgg ccatccgagt gatgatggag
      2410       2420       2430       2440       2450       2460
agcttcattg acacccagaa gttcagcgtc atgcggagta tgcgcaagac ttttgcccgg
      2470       2480       2490       2500       2510       2520
tatctctcct tccggcgaga taacaatgat ctgctgctct tcatactgaa gcagttggtg
      2530       2540       2550       2560       2570       2580
gctgagcagg tgacatatca acgcaaccgc tttggggccc agcaggacac cattgaaata
      2590       2600       2610       2620       2630       2640
cctgagaagg atctgatgga caaggccagg cagatcaata ttcacaacct ctctgccttc
      2650       2660       2670       2680       2690       2700
tacgacagcg acctcttcaa attcaacaag ttcagccgtg acctgaaacg caaactgatc
      2710
ctacagcagt tctga    

Last modification date: Oct. 2, 2011