Homo sapiens L. (human) [HSA]

FULL NAME: DNA replication licensing factor MCM2


DESCRIPTION:
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.

MISCELLANEOUS:
Early fractionation of eukaryotic MCM proteins yielded a variety of dimeric, trimeric and tetrameric complexes with unclear biological significance. Specifically a MCM467 subcomplex is shown to have in vitro helicase activity which is inhibited by the MCM2 subunit. The MCM2-7 hexamer is the proposed physiological active complex.


STRUCTURE SIMILARITY:
Contains 1 MCM domain.
Belongs to the MCM family.


CATALYTIC ACTIVITY:
ATP + H(2)O = ADP + phosphate.


POST-TRANSLATIONAL MODIFICATION:
Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.


RELATED PATHWAY(S):
DNA replication


Amino acids sequence

        10         20         30         40         50         60
MAESSESFTM ASSPAQRRRG NDPLTSSPGR SSRRTDALTS SPGRDLPPFE DESEGLLGTE
        70         80         90        100        110        120
GPLEEEEDGE ELIGDGMERD YRAIPELDAY EAEGLALDDE DVEELTASQR EAAERAMRQR
       130        140        150        160        170        180
DREAGRGLGR MRRGLLYDSD EEDEERPARK RRQVERATED GEEDEEMIES IENLEDLKGH
       190        200        210        220        230        240
SVREWVSMAG PRLEIHHRFK NFLRTHVDSH GHNVFKERIS DMCKENRESL VVNYEDLAAR
       250        260        270        280        290        300
EHVLAYFLPE APAELLQIFD EAALEVVLAM YPKYDRITNH IHVRISHLPL VEELRSLRQL
       310        320        330        340        350        360
HLNQLIRTSG VVTSCTGVLP QLSMVKYNCN KCNFVLGPFC QSQNQEVKPG SCPECQSAGP
       370        380        390        400        410        420
FEVNMEETIY QNYQRIRIQE SPGKVAAGRL PRSKDAILLA DLVDSCKPGD EIELTGIYHN
       430        440        450        460        470        480
NYDGSLNTAN GFPVFATVIL ANHVAKKDNK VAVGELTDED VKMITSLSKD QQIGEKIFAS
       490        500        510        520        530        540
IAPSIYGHED IKRGLALALF GGEPKNPGGK HKVRGDINVL LCGDPGTAKS QFLKYIEKVS
       550        560        570        580        590        600
SRAIFTTGQG ASAVGLTAYV QRHPVSREWT LEAGALVLAD RGVCLIDEFD KMNDQDRTSI
       610        620        630        640        650        660
HEAMEQQSIS ISKAGIVTSL QARCTVIAAA NPIGGRYDPS LTFSENVDLT EPIISRFDIL
       670        680        690        700        710        720
CVVRDTVDPV QDEMLARFVV GSHVRHHPSN KEEEGLANGS AAEPAMPNTY GVEPLPQEVL
       730        740        750        760        770        780
KKYIIYAKER VHPKLNQMDQ DKVAKMYSDL RKESMATGSI PITVRHIESM IRMAEAHARI
       790        800        810        820        830        840
HLRDYVIEDD VNMAIRVMLE SFIDTQKFSV MRSMRKTFAR YLSFRRDNNE LLLFILKQLV
       850        860        870        880        890        900
AEQVTYQRNR FGAQQDTIEV PEKDLVDKAR QINIHNLSAF YDSELFRMNK FSHDLKRKMI

LQQF     

Encoded by MCM2 gene

FULL NAME: minichromosome maintenance complex component 2


OTHER NAME(S):
BM28
CCNL1
CDCL1
D3S3194
KIAA0030
MGC10606
MITOTIN
cdc19


DESCRIPTION:
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. [provided by RefSeq, Jul 2008]


Nucleic acid sequence

        10         20         30         40         50         60
atggcggaat catcggaatc cttcaccatg gcatccagcc cggcccagcg tcggcgaggc
        70         80         90        100        110        120
aatgatcctc tcacctccag ccctggccga agctcccggc gtactgatgc cctcacctcc
       130        140        150        160        170        180
agccctggcc gtgaccttcc accatttgag gatgagtccg aggggctcct aggcacagag
       190        200        210        220        230        240
gggcccctgg aggaagaaga ggatggagag gagctcattg gagatggcat ggaaagggac
       250        260        270        280        290        300
taccgcgcca tcccagagct ggacgcctat gaggccgagg gactggctct ggatgatgag
       310        320        330        340        350        360
gacgtagagg agctgacggc cagtcagagg gaggcagcag agcgggccat gcggcagcgt
       370        380        390        400        410        420
gaccgggagg ctggccgggg cctgggccgc atgcgccgtg ggctcctgta tgacagcgat
       430        440        450        460        470        480
gaggaggacg aggagcgccc tgcccgcaag cgccgccagg tggagcgggc cacggaggac
       490        500        510        520        530        540
ggcgaggagg acgaggagat gatcgagagc atcgagaacc tggaggatct caaaggccac
       550        560        570        580        590        600
tctgtgcgcg agtgggtgag catggcgggc ccccggctgg agatccacca ccgcttcaag
       610        620        630        640        650        660
aacttcctgc gcactcacgt cgacagccac ggccacaacg tcttcaagga gcgcatcagc
       670        680        690        700        710        720
gacatgtgca aagagaaccg tgagagcctg gtggtgaact atgaggactt ggcagccagg
       730        740        750        760        770        780
gagcacgtgc tggcctactt cctgcctgag gcaccggcgg agctgctgca gatctttgat
       790        800        810        820        830        840
gaggctgccc tggaggtggt actggccatg taccccaagt acgaccgcat caccaaccac
       850        860        870        880        890        900
atccatgtcc gcatctccca cctgcctctg gtggaggagc tgcgctcgct gaggcagctg
       910        920        930        940        950        960
catctgaacc agctgatccg caccagtggg gtggtgacca gctgcactgg cgtcctgccc
       970        980        990       1000       1010       1020
cagctcagca tggtcaagta caactgcaac aagtgcaatt tcgtcctggg tcctttctgc
      1030       1040       1050       1060       1070       1080
cagtcccaga accaggaggt gaaaccaggc tcctgtcctg agtgccagtc ggccggcccc
      1090       1100       1110       1120       1130       1140
tttgaggtca acatggagga gaccatctat cagaactacc agcgtatccg aatccaggag
      1150       1160       1170       1180       1190       1200
agtccaggca aagtggcggc tggccggctg ccccgctcca aggacgccat tctcctcgca
      1210       1220       1230       1240       1250       1260
gatctggtgg acagctgcaa gccaggagac gagatagagc tgactggcat ctatcacaac
      1270       1280       1290       1300       1310       1320
aactatgatg gctccctcaa cactgccaat ggcttccctg tctttgccac tgtcatccta
      1330       1340       1350       1360       1370       1380
gccaaccacg tggccaagaa ggacaacaag gttgctgtag gggaactgac cgatgaagat
      1390       1400       1410       1420       1430       1440
gtgaagatga tcactagcct ctccaaggat cagcagatcg gagagaagat ctttgccagc
      1450       1460       1470       1480       1490       1500
attgctcctt ccatctatgg tcatgaagac atcaagagag gcctggctct ggccctgttc
      1510       1520       1530       1540       1550       1560
ggaggggagc ccaaaaaccc aggtggcaag cacaaggtac gtggtgatat caacgtgctc
      1570       1580       1590       1600       1610       1620
ttgtgcggag accctggcac agcgaagtcg cagtttctca agtatattga gaaagtgtcc
      1630       1640       1650       1660       1670       1680
agccgagcca tcttcaccac tggccagggg gcgtcggctg tgggcctcac ggcgtatgtc
      1690       1700       1710       1720       1730       1740
cagcggcacc ctgtcagcag ggagtggacc ttggaggctg gggccctggt tctggctgac
      1750       1760       1770       1780       1790       1800
cgaggagtgt gtctcattga tgaatttgac aagatgaatg accaggacag aaccagcatc
      1810       1820       1830       1840       1850       1860
catgaggcca tggagcaaca gagcatctcc atctcgaagg ctggcatcgt cacctccctg
      1870       1880       1890       1900       1910       1920
caggctcgct gcacggtcat tgctgccgcc aaccccatag gagggcgcta cgacccctcg
      1930       1940       1950       1960       1970       1980
ctgactttct ctgagaacgt ggacctcaca gagcccatca tctcacgctt tgacatcctg
      1990       2000       2010       2020       2030       2040
tgtgtggtga gggacaccgt ggacccagtc caggacgaga tgctggcccg cttcgtggtg
      2050       2060       2070       2080       2090       2100
ggcagccacg tcagacacca ccccagcaac aaggaggagg aggggctggc caatggcagc
      2110       2120       2130       2140       2150       2160
gctgctgagc ccgccatgcc caacacgtat ggcgtggagc ccctgcccca ggaggtcctg
      2170       2180       2190       2200       2210       2220
aagaagtaca tcatctacgc caaggagagg gtccacccga agctcaacca gatggaccag
      2230       2240       2250       2260       2270       2280
gacaaggtgg ccaagatgta cagtgacctg aggaaagaat ctatggcgac aggcagcatc
      2290       2300       2310       2320       2330       2340
cccattacgg tgcggcacat cgagtccatg atccgcatgg cggaggccca cgcgcgcatc
      2350       2360       2370       2380       2390       2400
catctgcggg actatgtgat cgaagacgac gtcaacatgg ccatccgcgt gatgctggag
      2410       2420       2430       2440       2450       2460
agcttcatag acacacagaa gttcagcgtc atgcgcagca tgcgcaagac ttttgcccgc
      2470       2480       2490       2500       2510       2520
tacctttcat tccggcgtga caacaatgag ctgttgctct tcatactgaa gcagttagtg
      2530       2540       2550       2560       2570       2580
gcagagcagg tgacatatca gcgcaaccgc tttggggccc agcaggacac tattgaggtc
      2590       2600       2610       2620       2630       2640
cctgagaagg acttggtgga taaggctcgt cagatcaaca tccacaacct ctctgcattt
      2650       2660       2670       2680       2690       2700
tatgacagtg agctcttcag gatgaacaag ttcagccacg acctgaaaag gaaaatgatc
      2710
ctgcagcagt tctga    

Last modification date: Oct. 2, 2011