Caenorhabditis elegans Maupas (nematode) [CEL]

FULL NAME: Flap endonuclease 1


DESCRIPTION:
Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA (By similarity). Can associate and cooperate with cps-6 to promote stepwise DNA fragmentation, utilizing the endonuclease activity of cps-6 and both of its own 5'-3' exonuclease activity and gap-dependent endonuclease activity. May play a critical role in switching the state of cells from DNA replication/repair to DNA degradation during apoptosis.

STRUCTURE SIMILARITY:
Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.


POST-TRANSLATIONAL MODIFICATION:
Phosphorylated. Phosphorylation upon DNA damage induces relocalization to the nuclear plasma (By similarity).


RELATED PATHWAY(S):
DNA replication
base excision repair (BER)
non-homologous end-joining (NHEJ)


Amino acids sequence

        10         20         30         40         50         60
MGIKGLSQVI ADNAPSAIKV NEMKAFFGRT VAIDASMCLY QFLIAVRQDG SQLQSEDGET
        70         80         90        100        110        120
TSHLMGMLNR TVRMFENGVK PVYVFDGKPP DMKGGELEKR SERRAEAEKA LTEAKEKGDV
       130        140        150        160        170        180
KEAEKFERRL VKVTKQQNDE AKRLLGLMGI PVVEAPCEAE AQCAHLVKAG KVFGTVTEDM
       190        200        210        220        230        240
DALTFGSTVL LRHFLAPVAK KIPIKEFNLS LALEEMKLSV EEFIDLCILL GCDYCGTIRG
       250        260        270        280        290        300
VGPKKAVELI RQHKNIETIL ENIDQNKYPP PEDWPYKRAR ELFLNPEVTK PEEVELTWKE
       310        320        330        340        350        360
ADVEGVIQFL CGEKNFNEER IRNALAKLKT SRKSGTQGRI DSFFGNSTKV TCVTAATKRK
       370        380
AEEAEKAKKG AKKGGPPKKR AK   

Encoded by crn-1 gene

FULL NAME: Cell-death-Related Nuclease


Nucleic acid sequence

        10         20         30         40         50         60
atgggaatta aaggactctc ccaagtgatt gctgataatg ctccctccgc gattaaagtc
        70         80         90        100        110        120
aacgagatga aggcgttctt cggaagaaca gtagcaatcg acgcatcaat gtgcctctac
       130        140        150        160        170        180
caattcctca ttgctgttcg tcaagatggt tcccagcttc aaagtgaaga tggagaaacc
       190        200        210        220        230        240
acaagtcact tgatgggaat gctgaatcgt acggttcgaa tgttcgagaa cggagtgaag
       250        260        270        280        290        300
cctgtctacg ttttcgacgg aaaaccaccg gatatgaagg gtggagagct tgaaaaacgt
       310        320        330        340        350        360
tccgagcgtc gtgctgaagc agaaaaagcg ctgactgagg ccaaggagaa gggagatgtt
       370        380        390        400        410        420
aaggaagcgg agaagttcga gagaagactc gtaaaagtca caaaacaaca gaacgacgag
       430        440        450        460        470        480
gcgaagcgtt tgctgggatt aatgggaatt ccagttgtcg aggctccatg cgaggcagaa
       490        500        510        520        530        540
gctcagtgtg ctcatctcgt gaaagctgga aaagtattcg gaactgtcac tgaggatatg
       550        560        570        580        590        600
gacgcgctga ccttcggctc cacggttctt ctcagacatt tcctggctcc agtcgccaag
       610        620        630        640        650        660
aaaatcccga tcaaggagtt caatctttct ttggctctcg aagaaatgaa gctaagcgtt
       670        680        690        700        710        720
gaggaattta ttgatttgtg tattcttctc ggctgtgact attgtggaac tattcgaggc
       730        740        750        760        770        780
gtcgggccta agaaggctgt tgagctgatc agacagcaca agaatattga gacgattctt
       790        800        810        820        830        840
gagaatatcg atcaaaataa atacccacca ccagaggatt ggccatacaa gcgtgctcgt
       850        860        870        880        890        900
gagcttttcc tcaatccaga agtgacaaaa ccggaagaag ttgaactgac atggaaggaa
       910        920        930        940        950        960
gccgatgtcg aaggtgtcat tcagttcttg tgcggagaga agaacttcaa tgaggagcgc
       970        980        990       1000       1010       1020
atccgaaacg cgttggcaaa actgaagact agccgtaaat ctggaacaca aggacgaatt
      1030       1040       1050       1060       1070       1080
gactcgttct tcggaaactc gaccaaggtt acatgtgtga cagctgcgac aaaacgaaag
      1090       1100       1110       1120       1130       1140
gcggaggaag ctgagaaggc gaagaaggga gcaaagaagg gtggaccacc gaagaagaga

gccaagtag     

Last modification date: Oct. 2, 2011