Caenorhabditis elegans Maupas (nematode) [CEL]

FULL NAME: DNA polymerase delta catalytic subunit


DESCRIPTION:
Possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Required with its accessory proteins (proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) or activator 1) for leading strand synthesis. Also involved in completing Okazaki fragments initiated by the DNA polymerase alpha/primase complex (By similarity).

MISCELLANEOUS:
In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis.


STRUCTURE SIMILARITY:
Belongs to the DNA polymerase type-B family.


SUBUNIT STRUCTURE:
Heterodimer with subunits of 125 kDa and 50 kDa. The 125 kDa subunit contains the polymerase active site and most likely the active site for the 3'-5' exonuclease activity (By similarity).


CATALYTIC ACTIVITY:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).


RELATED PATHWAY(S):
DNA replication
base excision repair (BER)
nucleotide excision repair (NER)
mismatch repair (MMR)
homologous recombination (HR)


Amino acids sequence

        10         20         30         40         50         60
MTSKRPGGSS FQPEVKRKRE SDEFEQCYVS RFENELPSVP TIDKTGWARP AVDKDLGISK
        70         80         90        100        110        120
SIACQILEVE TYHEDGSATS YDRTNVKLYG VTKSGNSICV IVTDYFPHFY FQAPQGFGVE
       130        140        150        160        170        180
HIGTAQSAIC NMVAAAKRRG GSGQAQLPGK VVDNLVHVEI VHGENLYYFR GADTKVPFVK
       190        200        210        220        230        240
VSGSTEALHK ARMELKNGVN LMGKGPVNVG NLYESNINVI VMFLAKTNIV GCGWIEIPAG
       250        260        270        280        290        300
KCRILSNSEK SSRCQIEVTV PVKNLIVHES DGEWAGIAPI RTLSLDIECI GRRGVFPEAI
       310        320        330        340        350        360
KDPIIQIANL VKIEGEAEPF VRNCFVLGTC APVVGSNIIQ CVNEKVLLEK WAEFVREVDP
       370        380        390        400        410        420
DIITGYNILN FDLPYILDRA KVLSLPQVSH LGRQKEKGSV VRDAAISSKQ MGSRVNKSID
       430        440        450        460        470        480
IHGRIIFDVL QVVLRDYKLR SYTLNSVSYQ FLSEQKEDVE HNIIPDLQRG DEQTRRRLAQ
       490        500        510        520        530        540
YCLKDAYLPL RLLDKLMSII NYIEMARVTG VPMNFLLTKG QQIKILSMML RRCKQNNFFL
       550        560        570        580        590        600
PVIEANSGDG EGYEGATVID PIRGFYNEPI ATLDFASLYP SIMIAHNLCY TTLLKSPQGV
       610        620        630        640        650        660
ENEDYIRTPS GQYFATKSKR RGLLPEILED ILAARKRAKN DMKNEKDEFK RMVYNGRQLA
       670        680        690        700        710        720
LKISANSVYG FTGATVGKLP CLEISQSVTA FGRKMIDMTK LEVERIYKKG ALDGKCPADA
       730        740        750        760        770        780
KVIYGDTDSV MVKFGVETVA QAMEIGLDAA KEVSKIFTPP IKLEFEKVYS PYLLINKKRY
       790        800        810        820        830        840
AGLYFTKPDV HDKMDCKGLE TVRRDNCPLV AKVLGVCLEK LLIERDQQSA LDFAKRTISD
       850        860        870        880        890        900
LLCNKIDISL LIISKELTKS GDKYQAKQAH VELAARMKKR DAGSAPRLGD RVPYVFVAAA
       910        920        930        940        950        960
KNVPAYERAE DPTFVLQNNI PLDTKHYLTN QLAKPLARIF EPILGDRAEK ILVEGEHTRV
       970        980        990       1000       1010       1020
RTVVQSKVGG LAAFTTKSAT CLGCKSVLPR AESENAVCKH CEPKLPTIFA SRMNTMHELE
      1030       1040       1050       1060       1070       1080
NHFGRLWTEC QNCAKTMQDK VNCSARDCPI YYMREKVRNE LSEASAVIER FGDPCFQAPT

K     

Encoded by gid:179652 gene


Nucleic acid sequence

        10         20         30         40         50         60
atgacctcca agcgtcctgg cgggagctcg tttcaaccag aagtgaagcg aaagcgtgaa
        70         80         90        100        110        120
agtgacgaat tcgagcagtg ttacgtttca agattcgaaa atgaacttcc atctgtacca
       130        140        150        160        170        180
acgattgata aaacaggatg ggccaggcca gcagtcgata aagatctcgg aattagcaaa
       190        200        210        220        230        240
agcattgcgt gtcagatctt agaggtcgaa acgtatcatg aagacggctc tgcaacatct
       250        260        270        280        290        300
tacgacagaa caaatgtgaa gttgtatggt gtaaccaaat ctggaaattc gatttgtgtc
       310        320        330        340        350        360
attgtgactg actactttcc acatttttat ttccaggcac cacaaggatt cggtgtcgag
       370        380        390        400        410        420
cacattggaa ctgcacaatc tgcaatttgc aatatggttg ctgcagcaaa acgccgaggt
       430        440        450        460        470        480
ggtagcggtc aagctcaatt gcctggaaaa gtagttgata atcttgttca tgtggagata
       490        500        510        520        530        540
gttcacggag agaatcttta ttacttccgc ggtgctgata cgaaagttcc ttttgtcaag
       550        560        570        580        590        600
gtgtcaggat ctacagaagc tcttcacaaa gcgcgaatgg aattgaaaaa tggagttaac
       610        620        630        640        650        660
ttgatgggaa agggtcctgt gaacgttggg aatttatacg agtcaaatat taatgtgatt
       670        680        690        700        710        720
gtcatgttct tggcgaaaac gaatattgtt ggatgtggct ggattgagat tcctgctgga
       730        740        750        760        770        780
aagtgccgaa ttctttcaaa cagtgaaaag tcgtctcgtt gtcaaataga agtcacagtt
       790        800        810        820        830        840
ccggtcaaaa acctgattgt tcatgaaagt gatggtgaat gggctggaat tgctcctatt
       850        860        870        880        890        900
cgtactttgt ctctggatat cgaatgtatt ggtcgacgtg gagtgtttcc cgaagcaatt
       910        920        930        940        950        960
aaagatccta ttattcaaat agctaatctt gtgaaaatcg aaggtgaagc agaaccattt
       970        980        990       1000       1010       1020
gttcgtaatt gtttcgttct tggaacatgt gcaccggtcg tcggttcaaa tatcattcaa
      1030       1040       1050       1060       1070       1080
tgtgtgaatg aaaaagttct tctcgaaaag tgggctgaat ttgttcgtga agtggatccc
      1090       1100       1110       1120       1130       1140
gacattatca ctggatataa cattctcaat tttgatcttc catacattct ggatcgtgct
      1150       1160       1170       1180       1190       1200
aaagttctat cactgccaca agtttctcat cttggtcgtc agaaagaaaa aggctcagtt
      1210       1220       1230       1240       1250       1260
gtaagagatg ctgcaatatc atcgaaacag atgggaagtc gagtgaacaa gagtatcgat
      1270       1280       1290       1300       1310       1320
attcacggaa gaattatttt tgatgtactt caagttgtac ttcgggatta caaactccga
      1330       1340       1350       1360       1370       1380
agttatacac tcaatagtgt ctcatatcaa tttttatccg aacaaaaaga agatgtggaa
      1390       1400       1410       1420       1430       1440
cataatatta ttcctgattt acaacgagga gatgaacaaa ctaggaggcg attggctcaa
      1450       1460       1470       1480       1490       1500
tattgtttga aagacgcata tcttcctctt cgtctgcttg ataaattaat gtcaattatt
      1510       1520       1530       1540       1550       1560
aattacatcg aaatggctag agtcaccgga gttcctatga atttcttact gacaaaaggt
      1570       1580       1590       1600       1610       1620
caacaaatca agattctatc aatgatgcta cgaagatgta agcagaacaa tttcttctta
      1630       1640       1650       1660       1670       1680
ccggtaattg aagccaattc tggggatggt gaaggatacg aaggtgctac tgtcattgat
      1690       1700       1710       1720       1730       1740
ccgattcgag gattttacaa tgaaccaatt gcaacacttg atttcgcttc tctatatcca
      1750       1760       1770       1780       1790       1800
tccattatga ttgctcataa tctctgttac acgacacttt taaaatcccc acaaggagtt
      1810       1820       1830       1840       1850       1860
gagaatgagg attatattcg gacaccatct ggccaatatt ttgcaaccaa atctaagcgc
      1870       1880       1890       1900       1910       1920
cgtgggcttc ttcccgagat tctcgaggat attctggctg caagaaaacg agcgaagaac
      1930       1940       1950       1960       1970       1980
gatatgaaaa acgagaaaga cgagttcaaa agaatggttt acaatggacg acaattggcg
      1990       2000       2010       2020       2030       2040
cttaaaatct ctgcaaattc tgtttatggt ttcactggtg caactgttgg aaagttgcca
      2050       2060       2070       2080       2090       2100
tgtctcgaga tttctcaatc agttacagct ttcggaagaa aaatgattga catgacgaaa
      2110       2120       2130       2140       2150       2160
ctcgaagtgg agagaattta caaaaaagga gctctcgatg gaaaatgtcc agcagatgcg
      2170       2180       2190       2200       2210       2220
aaagttattt atggcgatac agattctgta atggtgaaat tcggtgtcga aactgtagct
      2230       2240       2250       2260       2270       2280
caagcaatgg aaattggact cgatgctgct aaagaagtca gcaagatttt cactccgccg
      2290       2300       2310       2320       2330       2340
atcaaacttg aattcgagaa agtttactcc ccatatttac ttattaacaa aaagagatac
      2350       2360       2370       2380       2390       2400
gctggccttt acttcactaa accggatgtt catgacaaga tggattgtaa aggattagaa
      2410       2420       2430       2440       2450       2460
actgttcgcc gtgataactg ccctctggtt gcaaaagttc tcggtgtttg cctcgagaaa
      2470       2480       2490       2500       2510       2520
ttactaatag aaagagatca acaatctgct ttggattttg cgaaacggac aattagtgac
      2530       2540       2550       2560       2570       2580
ttgctctgca ataaaattga tatctcattg ttgattatca gtaaggaatt aacaaagtct
      2590       2600       2610       2620       2630       2640
ggagataaat atcaagcaaa gcaagctcac gttgagttgg cggctcgtat gaagaaaaga
      2650       2660       2670       2680       2690       2700
gacgctggaa gtgcaccaag acttggagat agagttccat atgtttttgt cgctgctgca
      2710       2720       2730       2740       2750       2760
aaaaatgttc cggcatacga acgagctgaa gatccaactt ttgtacttca aaataacatt
      2770       2780       2790       2800       2810       2820
ccattggaca ccaaacacta cttgactaat caactcgcca aaccacttgc cagaattttc
      2830       2840       2850       2860       2870       2880
gagcccatct tgggtgatag agcagaaaag attctggtgg aaggagaaca tacaagagtt
      2890       2900       2910       2920       2930       2940
cgtacagtcg ttcagagtaa agttggaggt cttgcagcgt tcacaacgaa atctgcaact
      2950       2960       2970       2980       2990       3000
tgtcttggtt gcaagagtgt tttaccaaga gcagaatcag agaacgcagt ttgcaaacac
      3010       3020       3030       3040       3050       3060
tgtgagccga agcttccaac gatttttgct tctagaatga acactatgca cgaacttgaa
      3070       3080       3090       3100       3110       3120
aaccattttg gtcgcctctg gaccgaatgc caaaattgtg caaagactat gcaagacaaa
      3130       3140       3150       3160       3170       3180
gtgaattgct cggctcgaga ctgtccgatc tactatatga gggaaaaagt tcgaaacgag
      3190       3200       3210       3220       3230       3240
ctatcggagg caagtgccgt catcgaacga tttggagatc cttgcttcca ggcacctact

aaatga     

Last modification date: Jan. 7, 2012