Homo sapiens L. (human) [HSA]

FULL NAME: Replication factor C subunit 1


DESCRIPTION:
Interacts with C-terminus of PCNA. 5' phosphate residue is required for binding of the N-terminal DNA-binding domain to duplex DNA, suggesting a role in recognition of non-primer template DNA structures during replication and/or repair.
The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA.

STRUCTURE SIMILARITY:
Belongs to the activator 1 large subunit family.
Contains 1 BRCT domain.


POST-TRANSLATIONAL MODIFICATION:
Phosphorylated upon DNA damage, probably by ATM or ATR.


RELATED PATHWAY(S):
DNA replication
nucleotide excision repair (NER)
mismatch repair (MMR)


Amino acids sequence

        10         20         30         40         50         60
MDIRKFFGVI PSGKKLVSET VKKNEKTKSD EETLKAKKGI KEIKVNSSRK EDDFKQKQPS
        70         80         90        100        110        120
KKKRIIYDSD SESEETLQVK NAKKPPEKLP VSSKPGKISR QDPVTYISET DEEDDFMCKK
       130        140        150        160        170        180
AASKSKENGR STNSHLGTSN MKKNEENTKT KNKPLSPIKL TPTSVLDYFG TGSVQRSNKK
       190        200        210        220        230        240
MVASKRKELS QNTDESGLND EAIAKQLQLD EDAELERQLH EDEEFARTLA MLDEEPKTKK
       250        260        270        280        290        300
ARKDTEAGET FSSVQANLSK AEKHKYPHKV KTAQVSDERK SYSPRKQSKY ESSKESQQHS
       310        320        330        340        350        360
KSSADKIGEV SSPKASSKLA IMKRKEESSY KEIEPVASKR KENAIKLKGE TKTPKKTKSS
       370        380        390        400        410        420
PAKKESVSPE DSEKKRTNYQ AYRSYLNREG PKALGSKEIP KGAENCLEGL IFVITGVLES
       430        440        450        460        470        480
IERDEAKSLI ERYGGKVTGN VSKKTNYLVM GRDSGQSKSD KAAALGTKII DEDGLLNLIR
       490        500        510        520        530        540
TMPGKKSKYE IAVETEMKKE SKLERTPQKN VQGKRKISPS KKESESKKSR PTSKRDSLAK
       550        560        570        580        590        600
TIKKETDVFW KSLDFKEQVA EETSGDSKAR NLADDSSENK VENLLWVDKY KPTSLKTIIG
       610        620        630        640        650        660
QQGDQSCANK LLRWLRNWQK SSSEDKKHAA KFGKFSGKDD GSSFKAALLS GPPGVGKTTT
       670        680        690        700        710        720
ASLVCQELGY SYVELNASDT RSKSSLKAIV AESLNNTSIK GFYSNGAASS VSTKHALIMD
       730        740        750        760        770        780
EVDGMAGNED RGGIQELIGL IKHTKIPIIC MCNDRNHPKI RSLVHYCFDL RFQRPRVEQI
       790        800        810        820        830        840
KGAMMSIAFK EGLKIPPPAM NEIILGANQD IRQVLHNLSM WCARSKALTY DQAKADSHRA
       850        860        870        880        890        900
KKDIKMGPFD VARKVFAAGE ETAHMSLVDK SDLFFHDYSI APLFVQENYI HVKPVAAGGD
       910        920        930        940        950        960
MKKHLMLLSR AADSICDGDL VDSQIRSKQN WSLLPAQAIY ASVLPGELMR GYMTQFPTFP
       970        980        990       1000       1010       1020
SWLGKHSSTG KHDRIVQDLA LHMSLRTYSS KRTVNMDYLS LLRDALVQPL TSQGVDGVQD
      1030       1040       1050       1060       1070       1080
VVALMDTYYL MKEDFENIME ISSWGGKPSP FSKLDPKVKA AFTRAYNKEA HLTPYSLQAI
      1090       1100       1110       1120       1130       1140
KASRHSTSPS LDSEYNEELN EDDSQSDEKD QDAIETDAMI KKKTKSSKPS KPEKDKEPRK

GKGKSSKK     

Encoded by RFC1 gene

FULL NAME: replication factor C (activator 1) 1, 145kDa


OTHER NAME(S):
A1
MGC51786
MHCBFB
PO-GA
RECC1
RFC
RFC140


DESCRIPTION:
This gene encodes the large subunit of replication factor C, a five subunit DNA polymerase accessory protein, which is a DNA-dependent ATPase required for eukaryotic DNA replication and repair. The large subunit acts as an activator of DNA polymerases, binds to the 3' end of primers, and promotes coordinated synthesis of both strands. It may also have a role in telomere stability. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Mar 2011]


Nucleic acid sequence

        10         20         30         40         50         60
atggacattc ggaaattctt tggagtaata ccaagtggaa agaaacttgt aagtgaaaca
        70         80         90        100        110        120
gtaaagaaga atgagaaaac aaagtctgat gaagaaactt taaaagcaaa gaaaggaata
       130        140        150        160        170        180
aaggaaatca aggtaaatag ctcccgtaaa gaggatgact tcaaacaaaa gcaaccaagc
       190        200        210        220        230        240
aagaaaaaga ggatcatcta tgattcagat tcagagtcag aggagacgtt gcaggtaaaa
       250        260        270        280        290        300
aatgccaaaa agccaccaga aaaactgcca gtatcttcta aacctggtaa aatttcacgg
       310        320        330        340        350        360
caggatcctg ttacatacat ttcagaaaca gatgaagaag atgactttat gtgtaagaag
       370        380        390        400        410        420
gcggcctcta aatcaaaaga gaatggaaga tctacaaata gtcatcttgg aacatcaaac
       430        440        450        460        470        480
atgaaaaaga atgaagaaaa cactaagacc aagaataagc ctttatcacc aataaaactt
       490        500        510        520        530        540
acacccacat cagtacttga ttattttgga actggaagtg tccaaagatc taataagaag
       550        560        570        580        590        600
atggtggcaa gcaaaagaaa agagctttca caaaatacag atgagtctgg attaaatgat
       610        620        630        640        650        660
gaagccatcg ccaagcaatt acagcttgat gaagatgcgg agctggagag gcagttgcat
       670        680        690        700        710        720
gaagatgaag agtttgccag aacattagcc atgttggatg aagaacccaa gaccaaaaag
       730        740        750        760        770        780
gctcgaaagg acacagaagc gggagaaacg ttttcatctg tccaagccaa tttaagtaaa
       790        800        810        820        830        840
gcagaaaaac ataaatatcc tcataaagta aaaacagcac aagtttcaga tgaaagaaag
       850        860        870        880        890        900
agctacagtc ctaggaagca aagtaaatat gaaagttcaa aagaatctca gcaacattcc
       910        920        930        940        950        960
aagtcatcag ctgacaaaat aggagaagtc tcttctccca aggccagttc taagctggca
       970        980        990       1000       1010       1020
attatgaaaa gaaaagaaga gagctcttat aaagaaatag agcctgtggc ctcaaaaaga
      1030       1040       1050       1060       1070       1080
aaagaaaatg ccattaaatt gaaaggagag acaaaaactc ctaagaaaac caaaagttct
      1090       1100       1110       1120       1130       1140
ccagctaaaa aagagtctgt aagtcctgaa gattctgaaa agaaacgcac taattatcaa
      1150       1160       1170       1180       1190       1200
gcttatcgaa gctacttaaa tcgagaaggt cccaaggctc tgggctccaa agaaatacca
      1210       1220       1230       1240       1250       1260
aagggagctg aaaattgctt ggaaggcctt atatttgtaa tcacaggcgt gctggagtct
      1270       1280       1290       1300       1310       1320
attgaacgag atgaggccaa gtctctaatt gaacgttatg ggggaaaagt aacaggaaat
      1330       1340       1350       1360       1370       1380
gtcagcaaga aaacaaatta tcttgtcatg ggtcgtgata gtggacagtc caagagtgat
      1390       1400       1410       1420       1430       1440
aaggccgcag ccttggggac aaaaattatt gatgaagatg gcctgttgaa tctgattcgg
      1450       1460       1470       1480       1490       1500
actatgccag gcaagaaatc caagtatgaa atagcagttg aaactgagat gaagaaagag
      1510       1520       1530       1540       1550       1560
tccaaactgg agagaacacc ccaaaaaaat gtccaaggaa aaagaaaaat tagtccatct
      1570       1580       1590       1600       1610       1620
aaaaaggaat cagaatctaa aaagagcagg ccgacttcca aaagggacag tttggcaaag
      1630       1640       1650       1660       1670       1680
acaataaaaa aggaaacaga tgtgttttgg aaaagcctgg atttcaagga gcaggtggct
      1690       1700       1710       1720       1730       1740
gaggagacaa gtggtgacag caaggctagg aatttggctg atgacagcag tgaaaacaaa
      1750       1760       1770       1780       1790       1800
gtggaaaatt tgctctgggt ggataaatat aagccaacct cgctcaagac cataattgga
      1810       1820       1830       1840       1850       1860
cagcaaggtg accagagctg tgccaacaaa ctcctacgct ggctccgaaa ctggcaaaag
      1870       1880       1890       1900       1910       1920
agttcttccg aagataaaaa acacgcaaag tttggtaaat tttccggcaa agatgatggc
      1930       1940       1950       1960       1970       1980
tctagtttta aagcagcgtt gctgtcaggc cctcctggtg ttggcaaaac caccacagct
      1990       2000       2010       2020       2030       2040
tccctggtgt gtcaggagtt gggatacagc tacgtggaac tgaatgcaag tgacacccgg
      2050       2060       2070       2080       2090       2100
agtaagagca gtttgaaggc gattgttgct gagtcactga acaataccag catcaaaggc
      2110       2120       2130       2140       2150       2160
ttttattcaa atggagcagc ctcttcagta agcacgaaac atgctctcat catggatgaa
      2170       2180       2190       2200       2210       2220
gtagatggca tggcaggcaa tgaggatagg ggaggaattc aggaattaat tggcctgata
      2230       2240       2250       2260       2270       2280
aaacatacta aaattcccat tatttgtatg tgcaatgata gaaatcatcc caagattcgc
      2290       2300       2310       2320       2330       2340
tctctggttc attattgttt tgatcttcgt tttcaaagac ctcgggttga acagattaag
      2350       2360       2370       2380       2390       2400
ggtgctatga tgtctattgc atttaaagaa ggtttaaaga ttccccctcc agctatgaat
      2410       2420       2430       2440       2450       2460
gaaataattt tgggagccaa tcaagatatc agacaggttt tacataatct gagtatgtgg
      2470       2480       2490       2500       2510       2520
tgtgcacgaa gtaaagcatt aacctatgac caggccaaag ctgattctca cagagccaaa
      2530       2540       2550       2560       2570       2580
aaggatatca aaatgggccc atttgatgtt gcccggaaag tgtttgcagc tggagaggag
      2590       2600       2610       2620       2630       2640
actgctcaca tgtcacttgt ggacaagtca gatctctttt ttcatgatta ttcaatagca
      2650       2660       2670       2680       2690       2700
cccctcttcg tccaggaaaa ttacatacac gtgaagcctg tagcagcagg gggtgacatg
      2710       2720       2730       2740       2750       2760
aaaaagcacc tgatgctttt aagcagagca gcagacagca tatgcgatgg tgacctagtg
      2770       2780       2790       2800       2810       2820
gacagccaga tccggagtaa gcaaaactgg agtcttctgc ctgcgcaggc catttatgcc
      2830       2840       2850       2860       2870       2880
agtgttcttc ctggagagtt gatgaggggg tacatgaccc agtttcccac cttcccaagc
      2890       2900       2910       2920       2930       2940
tggctgggga agcactcgtc tacaggcaaa catgatcgta ttgttcagga cctggccttg
      2950       2960       2970       2980       2990       3000
catatgagtc tcagaactta ctccagcaaa aggactgtaa acatggatta tctgtcgctt
      3010       3020       3030       3040       3050       3060
ctaagggatg cacttgtaca gcccttgacc tcacaaggag tagacggagt acaggatgtt
      3070       3080       3090       3100       3110       3120
gttgcactta tggacacata ttatttgatg aaagaagact ttgagaatat catggaaatc
      3130       3140       3150       3160       3170       3180
agcagctggg gtggcaaacc tagtcccttt tcaaagctgg atcccaaggt gaaagcagcc
      3190       3200       3210       3220       3230       3240
ttcacaagag cttacaataa ggaagcccac cttactccat actcacttca agctataaag
      3250       3260       3270       3280       3290       3300
gcatctagac acagcacaag cccatccctg gattcggaat acaatgaaga attaaatgaa
      3310       3320       3330       3340       3350       3360
gatgactctc aatctgatga gaaagaccaa gatgctatag aaactgatgc catgatcaag
      3370       3380       3390       3400       3410       3420
aaaaagacaa aatcttcaaa gccttcaaaa ccagaaaaag ataaggagcc cagaaaagga
      3430       3440
aaaggaaaaa gttcgaagaa atga   

Last modification date: Oct. 2, 2011