Schizo-saccharomyces pombe Lindner (fission yeast) [SPO]

FULL NAME: Flap endonuclease 1


DESCRIPTION:
Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA (By similarity).

STRUCTURE SIMILARITY:
Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.


POST-TRANSLATIONAL MODIFICATION:
Phosphorylated. Phosphorylation upon DNA damage induces relocalization to the nuclear plasma (By similarity).


RELATED PATHWAY(S):
DNA replication
base excision repair (BER)
non-homologous end-joining (NHEJ)


Amino acids sequence

        10         20         30         40         50         60
MGIKGLAQVL SEHAPASVKH NDIKNYFGRK VAIDASMSLY QFLIQVRSQD GQQLMNEQGE
        70         80         90        100        110        120
TTSHLMGMFY RTLRIVDNGI KPCFVFDGKP PTLKSGELAK RVARHQKARE DQEETKEVGT
       130        140        150        160        170        180
AEMVDRFAKR TVKVTRQHND EAKRLLELMG IPFVNAPCEA EAQCAALARS GKVYAAASED
       190        200        210        220        230        240
MDTLCFQAPV LLRHLTFSEQ RKEPISEYNI EKALNGLDMS VEQFVDLCIL LGCDYCEPIR
       250        260        270        280        290        300
GVGPARAVEL IRQYGTLDRF VKEADRSKYP IPEDWPYEDA RRLFLDAEVL PGEEIELKWK
       310        320        330        340        350        360
SPDADGIIQF LVKEKGFNED RVKLGINRLE KASKTIPQGR LDSFFKPVPS SPKKPVDTKS
       370        380
KGSAKRKRDS NKGGESKKKR    

Encoded by rad2 gene


Nucleic acid sequence

        10         20         30         40         50         60
atgggaatta aaggtttggc tcaagtacta agcgagcatg ccccagccag tgtcaaacat
        70         80         90        100        110        120
aatgatatta aaaattattt tggacggaaa gtggctatag atgcgtcaat gagtctttat
       130        140        150        160        170        180
caatttttaa ttcaagttcg cagtcaagat ggtcaacagc taatgaatga acaaggcgag
       190        200        210        220        230        240
actactagtc atttaatggg aatgttttat cgtacactcc gaattgtcga taatggaata
       250        260        270        280        290        300
aagccttgct ttgtctttga tggaaaacca ccaacattga agtcagggga attagctaaa
       310        320        330        340        350        360
cgtgtggccc gacatcaaaa agcacgagag gatcaagagg agacaaaaga ggttggtaca
       370        380        390        400        410        420
gctgaaatgg tggatcggtt tgccaaacgt acggtcaaag ttactcggca acataatgac
       430        440        450        460        470        480
gaagcaaaac gtttactaga gttaatggga attcccttcg ttaacgcacc ctgtgaagca
       490        500        510        520        530        540
gaagctcaat gtgccgcttt agcccgttca ggaaaagttt atgctgctgc ttctgaggac
       550        560        570        580        590        600
atggacacat tgtgcttcca agctcctgta cttttgagac acctgacatt tagtgagcaa
       610        620        630        640        650        660
agaaaggaac caattagtga gtataacata gaaaaagcat tgaatggttt agatatgtcg
       670        680        690        700        710        720
gtggagcagt ttgtagactt gtgtattctt ttgggttgtg actattgtga acctattcgg
       730        740        750        760        770        780
ggagttggcc ccgctagagc cgtcgagtta attaggcaat acgggactct cgatcgcttt
       790        800        810        820        830        840
gtcaaagagg cggatcgatc aaaatatcct attccggaag attggcctta tgaagatgcc
       850        860        870        880        890        900
agaagactgt ttttggatgc agaagtactt cctggtgaag agattgagtt gaaatggaaa
       910        920        930        940        950        960
agtcccgatg cagatggcat tatacaattt ctggttaagg aaaaaggttt caatgaagat
       970        980        990       1000       1010       1020
cgtgttaaat tggggatcaa tagactagaa aaagcctcaa agacaattcc acagggtcgt
      1030       1040       1050       1060       1070       1080
cttgattcct tttttaaacc agtcccttct tctcccaaaa aacctgtaga tacaaagagc
      1090       1100       1110       1120       1130       1140
aaaggatcag caaaaaggaa aagagattca aataagggtg gtgaaagcaa gaaaaagcgt

tga     

Last modification date: Oct. 2, 2011