Saccharomyces cerevisiae Meyen ex E.C. Hansen (budding yeast) [SCE]

FULL NAME: Deoxyribodipyrimidine photo-lyase, mitochondrial


DESCRIPTION:
Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ultraviolet radiation.

MISCELLANEOUS:
There are only 150-300 molecules of photolyase per yeast cell.
Present with 688 molecules/cell in log phase SD medium.


STRUCTURE SIMILARITY:
Contains 1 DNA photolyase domain.
Belongs to the DNA photolyase class-1 family.


CATALYTIC ACTIVITY:
Cyclobutadipyrimidine (in DNA) = 2 pyrimidine residues (in DNA).


RELATED PATHWAY(S):
direct reversal (DR)


RELATED DAMAGE:
CPD


Amino acids sequence

        10         20         30         40         50         60
MKRTVISSSN AYASKRSRLD IEHDFEQYHS LNKKYYPRPI TRTGANQFNN KSRAKPMEIV
        70         80         90        100        110        120
EKLQKKQKTS FENVSTVMHW FRNDLRLYDN VGLYKSVALF QQLRQKNAKA KLYAVYVINE
       130        140        150        160        170        180
DDWRAHMDSG WKLMFIMGAL KNLQQSLAEL HIPLLLWEFH TPKSTLSNSK EFVEFFKEKC
       190        200        210        220        230        240
MNVSSGTGTI ITANIEYQTD ELYRDIRLLE NEDHRLQLKY YHDSCIVAPG LITTDRGTNY
       250        260        270        280        290        300
SVFTPWYKKW VLYVNNYKKS TSEICHLHII EPLKYNETFE LKPFQYSLPD EFLQYIPKSK
       310        320        330        340        350        360
WCLPDVSEEA ALSRLKDFLG TKSSKYNNEK DMLYLGGTSG LSVYITTGRI STRLIVNQAF
       370        380        390        400        410        420
QSCNGQIMSK ALKDNSSTQN FIKEVAWRDF YRHCMCNWPY TSMGMPYRLD TLDIKWENNP
       430        440        450        460        470        480
VAFEKWCTGN TGIPIVDAIM RKLLYTGYIN NRSRMITASF LSKNLLIDWR WGERWFMKHL
       490        500        510        520        530        540
IDGDSSSNVG GWGFCSSTGI DAQPYFRVFN MDIQAKKYDP QMIFVKQWVP ELISSENKRP
       550        560
ENYPKPLVDL KHSRERALKV YKDAM   

Encoded by PHR1 gene


Nucleic acid sequence

        10         20         30         40         50         60
atgaaaagaa cggtaatatc ttcctcgaac gcatatgcta gtaagagatc tcggctggat
        70         80         90        100        110        120
atagaacatg attttgaaca ataccactcg ttgaacaaaa aatattatcc aaggccaatt
       130        140        150        160        170        180
actaggacag gcgcaaatca attcaataat aagtctcggg ctaaaccaat ggagattgtg
       190        200        210        220        230        240
gaaaaactgc aaaagaagca aaaaacgtcg tttgagaatg ttagcactgt catgcactgg
       250        260        270        280        290        300
tttcgaaatg atctacgttt atatgataat gtgggactgt acaaaagtgt tgcgctcttc
       310        320        330        340        350        360
cagcaattga ggcaaaaaaa cgcaaaagcc aaattatatg ctgtttatgt catcaatgaa
       370        380        390        400        410        420
gatgattgga gagcccatat ggatagcgga tggaaattga tgtttataat gggggcgtta
       430        440        450        460        470        480
aaaaatttgc agcagtcctt agccgaatta cacatacctc ttcttctgtg ggaattccac
       490        500        510        520        530        540
actccaaaaa gtaccttatc taattcaaaa gagttcgtgg agtttttcaa agaaaaatgt
       550        560        570        580        590        600
atgaatgtaa gttcaggaac aggtacgata atcactgcta atatagaata ccaaacagat
       610        620        630        640        650        660
gaactgtacc gtgatattag gctgttagaa aatgaagacc atagattgca attgaaatac
       670        680        690        700        710        720
taccacgact cttgcattgt tgctcctgga ttgatcacta ctgacagagg caccaactat
       730        740        750        760        770        780
tctgtgttta ctccatggta caaaaaatgg gtgctatatg taaataatta caaaaagagt
       790        800        810        820        830        840
acctctgaaa tttgtcattt gcatataatc gaaccattaa agtacaacga aacttttgaa
       850        860        870        880        890        900
ctgaagccat tccaatattc attaccggat gaattccttc aatatatacc taaatcaaaa
       910        920        930        940        950        960
tggtgcttgc cagacgtttc tgaagaagca gctttatcca gattaaaaga tttcctcggt
       970        980        990       1000       1010       1020
acaaaaagtt ctaagtacaa caatgaaaaa gacatgctat atttgggtgg aacctctggg
      1030       1040       1050       1060       1070       1080
ctaagtgtat atataactac tggtaggata agcacaagac ttatagtcaa ccaggcattt
      1090       1100       1110       1120       1130       1140
caatcatgca acggacaaat aatgtcaaaa gcactgaaag ataattcgtc cacccaaaat
      1150       1160       1170       1180       1190       1200
ttcatcaaag aagttgcatg gagagatttt tacagacatt gtatgtgcaa ctggccatat
      1210       1220       1230       1240       1250       1260
acctcaatgg gaatgcctta tcgattggac actttagata taaaatggga gaataaccct
      1270       1280       1290       1300       1310       1320
gtagcatttg aaaagtggtg tactggtaat acaggcattc ccatagtcga tgccataatg
      1330       1340       1350       1360       1370       1380
agaaaattac tgtacacagg ctatattaat aacagatcta gaatgatcac agcttctttt
      1390       1400       1410       1420       1430       1440
ctttctaaaa acttattaat agattggaga tggggggaac gctggtttat gaaacacttg
      1450       1460       1470       1480       1490       1500
atagacggtg attcgtcttc aaatgttggt ggctggggtt tttgttctag tacaggaatt
      1510       1520       1530       1540       1550       1560
gatgcccaac catattttag agtttttaat atggatatac aagcaaaaaa atatgaccca
      1570       1580       1590       1600       1610       1620
caaatgatat tcgtcaaaca atgggttccc gaattgattt cttccgaaaa taagcgtcca
      1630       1640       1650       1660       1670       1680
gaaaactatc caaaaccttt agttgattta aagcatagta gggagcgtgc tttgaaagtt
      1690
tataaagacg caatgtga    

Last modification date: Oct. 2, 2011