Mus musculus L. (mouse) [MMU]

FULL NAME: Methylated-DNA--protein-cysteine methyltransferase


DESCRIPTION:
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

MISCELLANEOUS:
This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocytalyst that acts repeatedly and over many reaction cycles.


STRUCTURE SIMILARITY:
Belongs to the MGMT family.


CATALYTIC ACTIVITY:
DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.


RELATED PATHWAY(S):
direct reversal (DR)


Amino acids sequence

        10         20         30         40         50         60
MAETCKMKYS VLDSPLGKME LSGCERGLHG IRLLSGKTPN TDPTEAPATP EVLGGPEGVP
        70         80         90        100        110        120
EPLVQCTAWL EAYFREPAAT EGLPLPALHH PVFQQDSFTR QVLWKLLKVV KFGETVSYQQ
       130        140        150        160        170        180
LAALAGNPKA ARAVGGAMRS NPVPILIPCH RVVRSDGAIG HYSGGGQAVK EWLLAHEGIP
       190        200        210
TGQPASKGLG LTGTWLKSSF ESTSSEPSGR N  

Encoded by Mgmt gene

FULL NAME: O-6-methylguanine-DNA methyltransferase


OTHER NAME(S):
AGT
AI267024
Agat
MGC107020


Nucleic acid sequence

        10         20         30         40         50         60
atggctgaga cctgcaaaat gaaatactca gtgttggaca gccctttggg gaagatggag
        70         80         90        100        110        120
ctgtctggct gtgagcgagg cctgcatggg atacggttgc tcagtgggaa gaccccaaac
       130        140        150        160        170        180
actgacccca cagaggcccc agctactcct gaggtgctcg gtgggccaga gggagttcca
       190        200        210        220        230        240
gagcctctgg tgcagtgcac agcctggctg gaagcctatt tccgtgaacc cgcagccaca
       250        260        270        280        290        300
gaggggcttc ccttgcctgc tctccatcac cctgtgttcc agcaagattc attcaccaga
       310        320        330        340        350        360
caggtgttat ggaagctgct gaaggttgtg aaattcggag aaacggtttc ttaccagcaa
       370        380        390        400        410        420
ttagcagccc tggcaggcaa ccccaaagcg gctcgtgcag taggaggagc aatgagaagc
       430        440        450        460        470        480
aatccggtcc ccatcctcat cccctgccac agggtggttc gcagtgacgg tgccatcggc
       490        500        510        520        530        540
cattactccg gaggagggca ggctgtgaag gagtggcttc tggcccatga gggcatcccg
       550        560        570        580        590        600
accggacagc cagcctccaa gggcttgggt ctgactggga cctggctcaa gtcatccttc
       610        620        630
gagtcgacca gctctgagcc gtctggccga aattga  

Last modification date: Oct. 2, 2011