Escherichia coli Migula (bacterium) K-12 MG1655 [ECO]

FULL NAME: DNA topoisomerase 3


DESCRIPTION:
The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase.

STRUCTURE SIMILARITY:
Contains 1 Toprim domain.
Belongs to the prokaryotic type I/III topoisomerase family.


CATALYTIC ACTIVITY:
ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.


PROTEIN TYPE(S):
DNA topoisomerase


RELATED PATHWAY(S):
DNA replication
prokaryotic nucleoid remodeling


Amino acids sequence

        10         20         30         40         50         60
MRLFIAEKPS LARAIADVLP KPHRKGDGFI ECGNGQVVTW CIGHLLEQAQ PDAYDSRYAR
        70         80         90        100        110        120
WNLADLPIVP EKWQLQPRPS VTKQLNVIKR FLHEASEIVH AGDPDREGQL LVDEVLDYLQ
       130        140        150        160        170        180
LAPEKRQQVQ RCLINDLNPQ AVERAIDRLR SNSEFVPLCV SALARARADW LYGINMTRAY
       190        200        210        220        230        240
TILGRNAGYQ GVLSVGRVQT PVLGLVVRRD EEIENFVAKD FFEVKAHIVT PADERFTAIW
       250        260        270        280        290        300
QPSEACEPYQ DEEGRLLHRP LAEHVVNRIS GQPAIVTSYN DKRESESAPL PFSLSALQIE
       310        320        330        340        350        360
AAKRFGLSAQ NVLDICQKLY ETHKLITYPR SDCRYLPEEH FAGRHAVMNA ISVHAPDLLP
       370        380        390        400        410        420
QPVVDPDIRN RCWDDKKVDA HHAIIPTARS SAINLTENEA KVYNLIARQY LMQFCPDAVF
       430        440        450        460        470        480
RKCVIELDIA KGKFVAKARF LAEAGWRTLL GSKERDEEND GTPLPVVAKG DELLCEKGEV
       490        500        510        520        530        540
VERQTQPPRH FTDATLLSAM TGIARFVQDK DLKKILRATD GLGTEATRAG IIELLFKRGF
       550        560        570        580        590        600
LTKKGRYIHS TDAGKALFHS LPEMATRPDM TAHWESVLTQ ISEKQCRYQD FMQPLVGTLY
       610        620        630        640        650
QLIDQAKRTP VRQFRGIVAP GSGGSADKKK AAPRKRSAKK SPPADEVGSG AIA

Encoded by topB gene


OTHER NAME(S):
ECK1761
JW1752
mutR


DESCRIPTION:
DNA topoisomerase III (Topo III) is a type I topoisomerase which can decatenate DNA; at high temperatures in vitro, the enzyme can also relax negative supercoils in DNA . [More information is available at EcoCyc: EG11014].


Nucleic acid sequence

        10         20         30         40         50         60
atgcggttgt ttattgccga aaaaccgagt ctggcgcgcg ccattgctga tgtcctgccc
        70         80         90        100        110        120
aaaccgcacc ggaaaggcga tggctttatc gagtgcggta atggtcaggt ggtgacctgg
       130        140        150        160        170        180
tgtatcggtc acctgcttga gcaggcgcag ccagacgcct acgacagccg ctatgcgcgc
       190        200        210        220        230        240
tggaatcttg cggatttgcc gattgtcccg gaaaagtggc aattacagcc ccgaccctcc
       250        260        270        280        290        300
gtgaccaaac aacttaacgt catcaaacgg ttcctgcatg aagccagcga aatcgttcac
       310        320        330        340        350        360
gccggggacc cggatcgtga agggcaattg ctggtggatg aagtgctgga ctatctgcaa
       370        380        390        400        410        420
ctggcaccgg aaaagcgcca gcaggtacag cgttgcttga taaacgacct gaacccgcag
       430        440        450        460        470        480
gcggttgagc gggcgatcga ccgtcttcgt tccaacagtg agtttgtacc gctgtgcgtt
       490        500        510        520        530        540
tctgcgctgg cgcgagcgcg tgccgactgg ctgtacggca tcaatatgac ccgtgcgtat
       550        560        570        580        590        600
accattctcg gtcgcaatgc cggttatcag ggcgtacttt ccgtgggacg cgtgcagacg
       610        620        630        640        650        660
cccgtgcttg ggctggtggt gcgccgcgat gaagagattg aaaacttcgt ggcgaaagac
       670        680        690        700        710        720
ttctttgaag tcaaagcaca tatcgtgaca cctgccgatg agcggtttac cgctatctgg
       730        740        750        760        770        780
caaccgagcg aagcgtgtga accgtaccag gatgaagaag ggcgcttgtt acatcgtcca
       790        800        810        820        830        840
ctggcggagc atgtggttaa ccgcattagt ggtcaaccgg ctattgtcac cagctataac
       850        860        870        880        890        900
gataaacggg aatcagaatc cgcgccgctg cctttttcgc tttcagcgtt gcagattgaa
       910        920        930        940        950        960
gcggcaaaac gttttggtct gagtgcgcag aacgtgcttg atatctgcca gaaactgtac
       970        980        990       1000       1010       1020
gaaacgcaca agctaatcac ttatccgcgt tctgattgtc gctatttgcc agaagaacat
      1030       1040       1050       1060       1070       1080
tttgccggac gccacgcggt gatgaatgcc atcagtgttc atgcaccgga tctgttgccg
      1090       1100       1110       1120       1130       1140
cagccagtgg tagatccaga tatacgcaac cgctgttggg atgacaaaaa ggtcgatgcg
      1150       1160       1170       1180       1190       1200
caccacgcca tcattccgac cgcacggagt tctgcgatca acctgacgga gaacgaagcg
      1210       1220       1230       1240       1250       1260
aaggtctata acctgattgc ccgtcagtat ctgatgcaat tctgcccgga tgcggtgttc
      1270       1280       1290       1300       1310       1320
cgcaagtgtg ttatcgaact ggacattgcc aaaggcaaat ttgtcgctaa agcgcgtttt
      1330       1340       1350       1360       1370       1380
cttgctgaag caggctggcg cacgctgtta ggcagcaaag agcgcgatga agaaaacgac
      1390       1400       1410       1420       1430       1440
ggcacgccac tgcctgtggt ggcgaaaggc gatgagttgc tgtgtgaaaa aggtgaagtg
      1450       1460       1470       1480       1490       1500
gtagagcggc aaacccagcc gccgcgccat tttaccgatg caacactgct ttcggcgatg
      1510       1520       1530       1540       1550       1560
accgggatcg cgcgctttgt gcaggataaa gatctgaaaa agatcctccg tgcgaccgat
      1570       1580       1590       1600       1610       1620
ggtctgggga cagaggcaac gcgtgccggg attattgaac tgttgttcaa gcgtggtttc
      1630       1640       1650       1660       1670       1680
ctgaccaaaa aagggcgcta tatccactcc accgacgccg gaaaagcgct attccattcg
      1690       1700       1710       1720       1730       1740
ctgccggaga tggcgacgcg accggacatg accgcgcact gggaatcggt gctgacgcaa
      1750       1760       1770       1780       1790       1800
atcagcgaaa agcagtgtcg ctatcaggac tttatgcagc cgctggtggg gacgctatat
      1810       1820       1830       1840       1850       1860
cagcttattg atcaagccaa acgtacgccg gtgcggcagt ttcgcggcat tgtggctccg
      1870       1880       1890       1900       1910       1920
ggcagtggtg gcagtgctga taagaaaaag gctgcaccgc gtaaacgtag tgcgaaaaaa
      1930       1940       1950       1960
agtccgccag cagatgaagt cggaagcggg gcgatagcgt aa 

Last modification date: Oct. 2, 2011