Escherichia coli Migula (bacterium) K-12 MG1655 [ECO]

FULL NAME: DNA gyrase subunit B


DESCRIPTION:
DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings.

STRUCTURE SIMILARITY:
Belongs to the type II topoisomerase family.


SUBUNIT STRUCTURE:
Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.


CATALYTIC ACTIVITY:
ATP-dependent breakage, passage and rejoining of double-stranded DNA.


ENZYME REGULATION:
Target of two classes of inhibitors, coumarins and quinolones. Coumarins bind to gyrB and are competitive inhibitors with respect to ATP. Quinolones bind DNA gyrase when the enzyme is complexed with DNA and trap the enzyme in an abortive ternary complex.


PROTEIN TYPE(S):
accessory protein
DNA topoisomerase


RELATED PATHWAY(S):
DNA replication
prokaryotic nucleoid remodeling


Amino acids sequence

        10         20         30         40         50         60
MSNSYDSSSI KVLKGLDAVR KRPGMYIGDT DDGTGLHHMV FEVVDNAIDE ALAGHCKEII
        70         80         90        100        110        120
VTIHADNSVS VQDDGRGIPT GIHPEEGVSA AEVIMTVLHA GGKFDDNSYK VSGGLHGVGV
       130        140        150        160        170        180
SVVNALSQKL ELVIQREGKI HRQIYEHGVP QAPLAVTGET EKTGTMVRFW PSLETFTNVT
       190        200        210        220        230        240
EFEYEILAKR LRELSFLNSG VSIRLRDKRD GKEDHFHYEG GIKAFVEYLN KNKTPIHPNI
       250        260        270        280        290        300
FYFSTEKDGI GVEVALQWND GFQENIYCFT NNIPQRDGGT HLAGFRAAMT RTLNAYMDKE
       310        320        330        340        350        360
GYSKKAKVSA TGDDAREGLI AVVSVKVPDP KFSSQTKDKL VSSEVKSAVE QQMNELLAEY
       370        380        390        400        410        420
LLENPTDAKI VVGKIIDAAR AREAARRARE MTRRKGALDL AGLPGKLADC QERDPALSEL
       430        440        450        460        470        480
YLVEGDSAGG SAKQGRNRKN QAILPLKGKI LNVEKARFDK MLSSQEVATL ITALGCGIGR
       490        500        510        520        530        540
DEYNPDKLRY HSIIIMTDAD VDGSHIRTLL LTFFYRQMPE IVERGHVYIA QPPLYKVKKG
       550        560        570        580        590        600
KQEQYIKDDE AMDQYQISIA LDGATLHTNA SAPALAGEAL EKLVSEYNAT QKMINRMERR
       610        620        630        640        650        660
YPKAMLKELI YQPTLTEADL SDEQTVTRWV NALVSELNDK EQHGSQWKFD VHTNAEQNLF
       670        680        690        700        710        720
EPIVRVRTHG VDTDYPLDHE FITGGEYRRI CTLGEKLRGL LEEDAFIERG ERRQPVASFE
       730        740        750        760        770        780
QALDWLVKES RRGLSIQRYK GLGEMNPEQL WETTMDPESR RMLRVTVKDA IAADQLFTTL
       790        800
MGDAVEPRRA FIEENALKAA NIDI   

Encoded by gyrB gene


OTHER NAME(S):
acrB
cou
ECK3691
himB
hisU
hopA
JW5625
nalC
parA
pcbA
pcpA
Cou


DESCRIPTION:
gyrB is an essential gene. [More information is available at EcoGene: EG10424]. The GyrB subunit of DNA gyrase is required for the ATPase activity of the enzyme. [More information is available at EcoCyc: EG10424].


Nucleic acid sequence

        10         20         30         40         50         60
atgtcgaatt cttatgactc ctccagtatc aaagtcctga aagggctgga tgcggtgcgt
        70         80         90        100        110        120
aagcgcccgg gtatgtatat cggcgacacg gatgacggca ccggtctgca ccacatggta
       130        140        150        160        170        180
ttcgaggtgg tagataacgc tatcgacgaa gcgctcgcgg gtcactgtaa agaaattatc
       190        200        210        220        230        240
gtcaccattc acgccgataa ctctgtctct gtacaggatg acgggcgcgg cattccgacc
       250        260        270        280        290        300
ggtattcacc cggaagaggg cgtatcggcg gcggaagtga tcatgaccgt tctgcacgca
       310        320        330        340        350        360
ggcggtaaat ttgacgataa ctcctataaa gtgtccggcg gtctgcacgg cgttggtgtt
       370        380        390        400        410        420
tcggtagtaa acgccctgtc gcaaaaactg gagctggtta tccagcgcga gggtaaaatt
       430        440        450        460        470        480
caccgtcaga tctacgaaca cggtgtaccg caggccccgc tggcggttac cggcgagact
       490        500        510        520        530        540
gaaaaaaccg gcaccatggt gcgtttctgg cccagcctcg aaaccttcac caatgtgacc
       550        560        570        580        590        600
gagttcgaat atgaaattct ggcgaaacgt ctgcgtgagt tgtcgttcct caactccggc
       610        620        630        640        650        660
gtttccattc gtctgcgcga caagcgcgac ggcaaagaag accacttcca ctatgaaggc
       670        680        690        700        710        720
ggcatcaagg cgttcgttga atatctgaac aagaacaaaa cgccgatcca cccgaatatc
       730        740        750        760        770        780
ttctacttct ccactgaaaa agacggtatt ggcgtcgaag tggcgttgca gtggaacgat
       790        800        810        820        830        840
ggcttccagg aaaacatcta ctgctttacc aacaacattc cgcagcgtga cggcggtact
       850        860        870        880        890        900
cacctggcag gcttccgtgc ggcgatgacc cgtaccctga acgcctacat ggacaaagaa
       910        920        930        940        950        960
ggctacagca aaaaagccaa agtcagcgcc accggtgacg atgcgcgtga aggcctgatt
       970        980        990       1000       1010       1020
gcggtcgttt ccgtgaaagt gccggacccg aaattctcct cccagaccaa agacaaactg
      1030       1040       1050       1060       1070       1080
gtttcttctg aggtgaaatc ggcggttgaa cagcagatga acgaactgct ggcagaatac
      1090       1100       1110       1120       1130       1140
ctgctggaaa acccaaccga cgcgaaaatc gtggttggca aaattatcga tgctgcccgt
      1150       1160       1170       1180       1190       1200
gcccgtgaag cggcgcgtcg cgcgcgtgaa atgacccgcc gtaaaggtgc gctcgactta
      1210       1220       1230       1240       1250       1260
gcgggcctgc cgggcaaact ggcagactgc caggaacgcg atccggcgct ttccgaactg
      1270       1280       1290       1300       1310       1320
tacctggtgg aaggggactc cgcgggcggc tctgcgaagc aggggcgtaa ccgcaagaac
      1330       1340       1350       1360       1370       1380
caggcgattc tgccgctgaa gggtaaaatc ctcaacgtcg agaaagcgcg cttcgataag
      1390       1400       1410       1420       1430       1440
atgctctctt ctcaggaagt ggcgacgctt atcaccgcgc ttggctgtgg tatcggtcgt
      1450       1460       1470       1480       1490       1500
gacgagtaca acccggacaa actgcgttat cacagcatca tcatcatgac cgatgcggac
      1510       1520       1530       1540       1550       1560
gtcgacggct cgcacattcg tacgctgctg ttgaccttct tctatcgtca gatgccggaa
      1570       1580       1590       1600       1610       1620
atcgttgaac gcggtcacgt ctacatcgct cagccgccgc tgtacaaagt gaagaaaggc
      1630       1640       1650       1660       1670       1680
aagcaggaac agtacattaa agacgacgaa gcgatggatc agtaccagat ctctatcgcg
      1690       1700       1710       1720       1730       1740
ctggacggcg caacgctgca caccaacgcc agtgcaccgg cattggctgg cgaagcgtta
      1750       1760       1770       1780       1790       1800
gagaaactgg tatctgagta caacgcgacg cagaaaatga tcaatcgtat ggagcgtcgt
      1810       1820       1830       1840       1850       1860
tatccgaaag caatgctgaa agagcttatc tatcagccga cgttgacgga agctgacctt
      1870       1880       1890       1900       1910       1920
tctgatgagc agaccgttac ccgctgggtg aacgcgctgg tcagcgaact gaacgacaaa
      1930       1940       1950       1960       1970       1980
gaacagcacg gcagccagtg gaagtttgat gttcacacca atgctgagca aaacctgttc
      1990       2000       2010       2020       2030       2040
gagccgattg ttcgcgtgcg tacccacggt gtggatactg actatccgct ggatcacgag
      2050       2060       2070       2080       2090       2100
tttatcaccg gtggcgaata tcgtcgtatc tgcacgctgg gtgagaaact gcgtggcttg
      2110       2120       2130       2140       2150       2160
ctggaagaag atgcgtttat cgaacgtggc gagcgtcgtc agccggtagc cagcttcgag
      2170       2180       2190       2200       2210       2220
caggcgctgg actggctggt gaaagagtcc cgtcgcggcc tctccatcca gcgttataaa
      2230       2240       2250       2260       2270       2280
ggtctgggcg agatgaaccc ggaacagctg tgggaaacca ctatggaccc ggaaagtcgt
      2290       2300       2310       2320       2330       2340
cgtatgctgc gcgttaccgt taaagatgcg attgctgccg accagttgtt caccacgctg
      2350       2360       2370       2380       2390       2400
atgggcgacg ccgttgaacc gcgccgtgcg tttattgaag agaacgccct gaaagcggcg
      2410
aatatcgata tttaa    

Last modification date: Oct. 5, 2011