Escherichia coli Migula (bacterium) K-12 MG1655 [ECO]

FULL NAME: DNA gyrase subunit A


DESCRIPTION:
DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings.

MISCELLANEOUS:
When the enzyme transiently cleaves DNA a phosphotyrosine bond is formed between GyrA and DNA. This enzyme-DNA intermediate is the target of a number of topoisomerase poisons, including toxin CcdB.


STRUCTURE SIMILARITY:
Belongs to the topoisomerase GyrA/ParC subunit family.


CATALYTIC ACTIVITY:
ATP-dependent breakage, passage and rejoining of double-stranded DNA.


PROTEIN TYPE(S):
DNA topoisomerase


RELATED PATHWAY(S):
DNA replication
prokaryotic nucleoid remodeling


Amino acids sequence

        10         20         30         40         50         60
MSDLAREITP VNIEEELKSS YLDYAMSVIV GRALPDVRDG LKPVHRRVLY AMNVLGNDWN
        70         80         90        100        110        120
KAYKKSARVV GDVIGKYHPH GDSAVYDTIV RMAQPFSLRY MLVDGQGNFG SIDGDSAAAM
       130        140        150        160        170        180
RYTEIRLAKI AHELMADLEK ETVDFVDNYD GTEKIPDVMP TKIPNLLVNG SSGIAVGMAT
       190        200        210        220        230        240
NIPPHNLTEV INGCLAYIDD EDISIEGLME HIPGPDFPTA AIINGRRGIE EAYRTGRGKV
       250        260        270        280        290        300
YIRARAEVEV DAKTGRETII VHEIPYQVNK ARLIEKIAEL VKEKRVEGIS ALRDESDKDG
       310        320        330        340        350        360
MRIVIEVKRD AVGEVVLNNL YSQTQLQVSF GINMVALHHG QPKIMNLKDI IAAFVRHRRE
       370        380        390        400        410        420
VVTRRTIFEL RKARDRAHIL EALAVALANI DPIIELIRHA PTPAEAKTAL VANPWQLGNV
       430        440        450        460        470        480
AAMLERAGDD AARPEWLEPE FGVRDGLYYL TEQQAQAILD LRLQKLTGLE HEKLLDEYKE
       490        500        510        520        530        540
LLDQIAELLR ILGSADRLME VIREELELVR EQFGDKRRTE ITANSADINL EDLITQEDVV
       550        560        570        580        590        600
VTLSHQGYVK YQPLSEYEAQ RRGGKGKSAA RIKEEDFIDR LLVANTHDHI LCFSSRGRVY
       610        620        630        640        650        660
SMKVYQLPEA TRGARGRPIV NLLPLEQDER ITAILPVTEF EEGVKVFMAT ANGTVKKTVL
       670        680        690        700        710        720
TEFNRLRTAG KVAIKLVDGD ELIGVDLTSG EDEVMLFSAE GKVVRFKESS VRAMGCNTTG
       730        740        750        760        770        780
VRGIRLGEGD KVVSLIVPRG DGAILTATQN GYGKRTAVAE YPTKSRATKG VISIKVTERN
       790        800        810        820        830        840
GLVVGAVQVD DCDQIMMITD AGTLVRTRVS EISIVGRNTQ GVILIRTAED ENVVGLQRVA
       850        860        870
EPVDEEDLDT IDGSAAEGDD EIAPEVDVDD EPEEE  

Encoded by gyrA gene


OTHER NAME(S):
ECK2223
hisW
JW2225
nalA
nfxA
norA
parD


DESCRIPTION:
gyrA is an essential gene. [More information is available at EcoGene: EG10423]. DNA gyrase is one of two essential members of the type II topoisomerase family in Escherichia coli. [More information is available at EcoCyc: EG10423].


Nucleic acid sequence

        10         20         30         40         50         60
atgagcgacc ttgcgagaga aattacaccg gtcaacattg aggaagagct gaagagctcc
        70         80         90        100        110        120
tatctggatt atgcgatgtc ggtcattgtt ggccgtgcgc tgccagatgt ccgagatggc
       130        140        150        160        170        180
ctgaagccgg tacaccgtcg cgtactttac gccatgaacg tactaggcaa tgactggaac
       190        200        210        220        230        240
aaagcctata aaaaatctgc ccgtgtcgtt ggtgacgtaa tcggtaaata ccatccccat
       250        260        270        280        290        300
ggtgactcgg cggtctatga cacgatcgtc cgcatggcgc agccattctc gctgcgttat
       310        320        330        340        350        360
atgctggtag acggtcaggg taacttcggt tctatcgacg gcgactctgc ggcggcaatg
       370        380        390        400        410        420
cgttatacgg aaatccgtct ggcgaaaatt gcccatgaac tgatggccga tctcgaaaaa
       430        440        450        460        470        480
gagacggtcg atttcgttga taactatgac ggcacggaaa aaattccgga cgtcatgcca
       490        500        510        520        530        540
accaaaattc ctaacctgct ggtgaacggt tcttccggta tcgccgtagg tatggcaacc
       550        560        570        580        590        600
aacatcccgc cgcacaacct gacggaagtc atcaacggtt gtctggcgta tattgatgat
       610        620        630        640        650        660
gaagacatca gcattgaagg gctgatggaa cacatcccgg ggccggactt cccgacggcg
       670        680        690        700        710        720
gcaatcatta acggtcgtcg cggtattgaa gaagcttacc gtaccggtcg cggcaaggtg
       730        740        750        760        770        780
tatatccgcg ctcgcgcaga agtggaagtt gacgccaaaa ccggtcgtga aaccattatc
       790        800        810        820        830        840
gtccacgaaa ttccgtatca ggtaaacaaa gcgcgcctga tcgagaagat tgcggaactg
       850        860        870        880        890        900
gtaaaagaaa aacgcgtgga aggcatcagc gcgctgcgtg acgagtctga caaagacggt
       910        920        930        940        950        960
atgcgcatcg tgattgaagt gaaacgcgat gcggtcggtg aagttgtgct caacaacctc
       970        980        990       1000       1010       1020
tactcccaga cccagttgca ggtttctttc ggtatcaaca tggtggcatt gcaccatggt
      1030       1040       1050       1060       1070       1080
cagccgaaga tcatgaacct gaaagacatc atcgcggcgt ttgttcgtca ccgccgtgaa
      1090       1100       1110       1120       1130       1140
gtggtgaccc gtcgtactat tttcgaactg cgtaaagctc gcgatcgtgc tcatatcctt
      1150       1160       1170       1180       1190       1200
gaagcattag ccgtggcgct ggcgaacatc gacccgatca tcgaactgat ccgtcatgcg
      1210       1220       1230       1240       1250       1260
ccgacgcctg cagaagcgaa aactgcgctg gttgctaatc cgtggcagct gggcaacgtt
      1270       1280       1290       1300       1310       1320
gccgcgatgc tcgaacgtgc tggcgacgat gctgcgcgtc cggaatggct ggagccagag
      1330       1340       1350       1360       1370       1380
ttcggcgtgc gtgatggtct gtactacctg accgaacagc aagctcaggc gattctggat
      1390       1400       1410       1420       1430       1440
ctgcgtttgc agaaactgac cggtcttgag cacgaaaaac tgctcgacga atacaaagag
      1450       1460       1470       1480       1490       1500
ctgctggatc agatcgcgga actgttgcgt attcttggta gcgccgatcg tctgatggaa
      1510       1520       1530       1540       1550       1560
gtgatccgtg aagagctgga gctggttcgt gaacagttcg gtgacaaacg tcgtactgaa
      1570       1580       1590       1600       1610       1620
atcaccgcca acagcgcaga catcaacctg gaagatctga tcacccagga agatgtggtc
      1630       1640       1650       1660       1670       1680
gtgacgctct ctcaccaggg ctacgttaag tatcagccgc tttctgaata cgaagcgcag
      1690       1700       1710       1720       1730       1740
cgtcgtggcg ggaaaggtaa atctgccgca cgtattaaag aagaagactt tatcgaccga
      1750       1760       1770       1780       1790       1800
ctgctggtgg cgaacactca cgaccatatt ctgtgcttct ccagccgtgg tcgcgtctat
      1810       1820       1830       1840       1850       1860
tcgatgaaag tttatcagtt gccggaagcc actcgtggcg cgcgcggtcg tccgatcgtc
      1870       1880       1890       1900       1910       1920
aacctgctgc cgctggagca ggacgaacgt atcactgcga tcctgccagt gaccgagttt
      1930       1940       1950       1960       1970       1980
gaagaaggcg tgaaagtctt catggcgacc gctaacggta ccgtgaagaa aactgtcctc
      1990       2000       2010       2020       2030       2040
accgagttca accgtctgcg taccgccggt aaagtggcga tcaaactggt tgacggcgat
      2050       2060       2070       2080       2090       2100
gagctgatcg gcgttgacct gaccagcggc gaagacgaag taatgctgtt ctccgctgaa
      2110       2120       2130       2140       2150       2160
ggtaaagtgg tgcgctttaa agagtcttct gtccgtgcga tgggctgcaa caccaccggt
      2170       2180       2190       2200       2210       2220
gttcgcggta ttcgcttagg tgaaggcgat aaagtcgtct ctctgatcgt gcctcgtggc
      2230       2240       2250       2260       2270       2280
gatggcgcaa tcctcaccgc aacgcaaaac ggttacggta aacgtaccgc agtggcggaa
      2290       2300       2310       2320       2330       2340
tacccaacca agtcgcgtgc gacgaaaggg gttatctcca tcaaggttac cgaacgtaac
      2350       2360       2370       2380       2390       2400
ggtttagttg ttggcgcggt acaggtagat gactgcgacc agatcatgat gatcaccgat
      2410       2420       2430       2440       2450       2460
gccggtacgc tggtacgtac tcgcgtttcg gaaatcagca tcgtgggccg taacacccag
      2470       2480       2490       2500       2510       2520
ggcgtgatcc tcatccgtac tgcggaagat gaaaacgtag tgggtctgca acgtgttgct
      2530       2540       2550       2560       2570       2580
gaaccggttg acgaggaaga tctggatacc atcgacggca gtgccgcgga aggggacgat
      2590       2600       2610       2620
gaaatcgctc cggaagtgga cgttgacgac gagccagaag aagaataa 

Last modification date: Oct. 2, 2011