Escherichia coli Migula (bacterium) K-12 MG1655 [ECO]

FULL NAME: DNA topoisomerase 1


DESCRIPTION:
The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.

MISCELLANEOUS:
When a topoisomerase transiently breaks a DNA backbone bond, it simultaneously forms a protein-DNA link, in which a tyrosyl oxygen in the enzyme is joined to a DNA phosphorus at one end of the enzyme-severed DNA strand.


STRUCTURE SIMILARITY:
Contains 1 Toprim domain.
Belongs to the prokaryotic type I/III topoisomerase family.


CATALYTIC ACTIVITY:
ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.


PROTEIN TYPE(S):
DNA topoisomerase


RELATED PATHWAY(S):
DNA replication
prokaryotic nucleoid remodeling


Amino acids sequence

        10         20         30         40         50         60
MGKALVIVES PAKAKTINKY LGSDYVVKSS VGHIRDLPTS GSAAKKSADS TSTKTAKKPK
        70         80         90        100        110        120
KDERGALVNR MGVDPWHNWE AHYEVLPGKE KVVSELKQLA EKADHIYLAT DLDREGEAIA
       130        140        150        160        170        180
WHLREVIGGD DARYSRVVFN EITKNAIRQA FNKPGELNID RVNAQQARRF MDRVVGYMVS
       190        200        210        220        230        240
PLLWKKIARG LSAGRVQSVA VRLVVERERE IKAFVPEEFW EVDASTTTPS GEALALQVTH
       250        260        270        280        290        300
QNDKPFRPVN KEQTQAAVSL LEKARYSVLE REDKPTTSKP GAPFITSTLQ QAASTRLGFG
       310        320        330        340        350        360
VKKTMMMAQR LYEAGYITYM RTDSTNLSQD AVNMVRGYIS DNFGKKYLPE SPNQYASKEN
       370        380        390        400        410        420
SQEAHEAIRP SDVNVMAESL KDMEADAQKL YQLIWRQFVA CQMTPAKYDS TTLTVGAGDF
       430        440        450        460        470        480
RLKARGRILR FDGWTKVMPA LRKGDEDRIL PAVNKGDALT LVELTPAQHF TKPPARFSEA
       490        500        510        520        530        540
SLVKELEKRG IGRPSTYASI ISTIQDRGYV RVENRRFYAE KMGEIVTDRL EENFRELMNY
       550        560        570        580        590        600
DFTAQMENSL DQVANHEAEW KAVLDHFFSD FTQQLDKAEK DPEEGGMRPN QMVLTSIDCP
       610        620        630        640        650        660
TCGRKMGIRT ASTGVFLGCS GYALPPKERC KTTINLVPEN EVLNVLEGED AETNALRAKR
       670        680        690        700        710        720
RCPKCGTAMD SYLIDPKRKL HVCGNNPTCD GYEIEEGEFR IKGYDGPIVE CEKCGSEMHL
       730        740        750        760        770        780
KMGRFGKYMA CTNEECKNTR KILRNGEVAP PKEDPVPLPE LPCEKSDAYF VLRDGAAGVF
       790        800        810        820        830        840
LAANTFPKSR ETRAPLVEEL YRFRDRLPEK LRYLADAPQQ DPEGNKTMVR FSRKTKQQYV
       850        860
SSEKDGKATG WSAFYVDGKW VEGKK   

Encoded by topA gene


OTHER NAME(S):
asuA?
ECK1268
JW1266
supX


DESCRIPTION:
Topoisomerase I is a type IA topoisomerase which is responsible for relaxing negative supercoils in DNA. [More information is available at EcoCyc: EG11013].


Nucleic acid sequence

        10         20         30         40         50         60
atgggtaaag ctcttgtcat cgttgagtcc ccggcaaaag ccaaaacgat caacaagtat
        70         80         90        100        110        120
ctgggtagtg actacgtggt gaaatccagc gtcggtcaca tccgcgattt gccgaccagt
       130        140        150        160        170        180
ggctcagctg ccaaaaagag tgccgactct acctccacca agacggctaa aaagcctaaa
       190        200        210        220        230        240
aaggatgaac gtggcgctct cgtcaaccgt atgggggttg acccgtggca caattgggag
       250        260        270        280        290        300
gcgcactatg aagtgttgcc tggtaaagag aaggtcgtct ctgaactgaa acaactggct
       310        320        330        340        350        360
gaaaaagccg accacatcta tctcgcaacc gaccttgacc gcgaagggga agccattgca
       370        380        390        400        410        420
tggcacctgc gggaagtgat tgggggtgat gatgcgcgct atagccgagt ggtgtttaac
       430        440        450        460        470        480
gaaattacta aaaacgcgat ccgccaggca tttaacaaac cgggtgagct gaatattgat
       490        500        510        520        530        540
cgtgttaatg cccagcaggc gcgtcgcttt atggaccgcg tggtggggta tatggtttcg
       550        560        570        580        590        600
ccgctgctat ggaaaaagat cgctcgtggc ctgtctgccg gtcgtgtgca gtcggtggcg
       610        620        630        640        650        660
gttcgcctgg tggtcgagcg tgagcgtgaa attaaagcgt tcgtgccgga agagttctgg
       670        680        690        700        710        720
gaagtcgatg ccagcacgac cacgccatct ggtgaagcgt tggcgttaca ggtgactcat
       730        740        750        760        770        780
cagaacgaca aaccgttccg tccggtcaac aaagaacaaa ctcaggctgc ggtaagtctg
       790        800        810        820        830        840
ctggaaaaag cgcgctacag cgtgctggaa cgtgaagaca aaccgacaac cagtaaacct
       850        860        870        880        890        900
ggcgctcctt ttattacctc tacgctgcaa caagctgcca gcacccgtct tggatttggc
       910        920        930        940        950        960
gtgaaaaaaa ccatgatgat ggcgcagcgt ttgtatgaag caggctatat cacttacatg
       970        980        990       1000       1010       1020
cgtaccgact ccactaacct gagtcaggac gcggtaaata tggttcgcgg ttatatcagc
      1030       1040       1050       1060       1070       1080
gataattttg gtaagaaata tctgccggaa agtccgaatc agtacgccag caaagaaaac
      1090       1100       1110       1120       1130       1140
tcacaggaag cgcacgaagc gattcgtcct tctgacgtca atgtgatggc ggaatcgctg
      1150       1160       1170       1180       1190       1200
aaggatatgg aagcagatgc gcagaaactg taccagttaa tctggcgtca gttcgttgcc
      1210       1220       1230       1240       1250       1260
tgccagatga ccccagcgaa atatgactcc acgacgctga ccgttggtgc gggcgatttc
      1270       1280       1290       1300       1310       1320
cgcctgaaag cacgcggtcg tattttgcgt tttgatggct ggacaaaagt gatgcctgcg
      1330       1340       1350       1360       1370       1380
ttgcgtaaag gcgatgaaga tcgcatctta ccagcagtta ataaaggcga tgctctgacg
      1390       1400       1410       1420       1430       1440
ctcgttgaac ttacaccagc ccagcacttt accaagccgc cagcccgttt cagtgaagca
      1450       1460       1470       1480       1490       1500
tcgctggtta aagagctgga aaaacgcggt atcggtcgtc cgtctaccta tgcgtcgatc
      1510       1520       1530       1540       1550       1560
atttcgacca ttcaggatcg tggctacgtg cgagtagaaa atcgtcgttt ctatgcggaa
      1570       1580       1590       1600       1610       1620
aaaatgggcg aaatcgtcac cgatcgcctt gaagaaaatt tccgcgagtt aatgaactac
      1630       1640       1650       1660       1670       1680
gactttaccg cgcagatgga aaacagcctc gaccaggtgg caaatcacga agcagagtgg
      1690       1700       1710       1720       1730       1740
aaagctgtac tggatcactt cttctcggat ttcacccagc agttagataa agctgaaaaa
      1750       1760       1770       1780       1790       1800
gatccggaag agggtggtat gcgcccgaac cagatggttc tgaccagcat tgactgcccg
      1810       1820       1830       1840       1850       1860
acttgtggtc gcaaaatggg gattcgcaca gcgagcaccg gggtattcct tggctgttct
      1870       1880       1890       1900       1910       1920
ggctatgcgc tgccgccgaa agagcgttgc aaaaccacca ttaacctggt gccggaaaac
      1930       1940       1950       1960       1970       1980
gaagtgctga acgtgctgga aggcgaagat gctgaaacca acgcgctgcg cgcaaaacgt
      1990       2000       2010       2020       2030       2040
cgttgcccga aatgcggcac ggcgatggac agctatctca tcgatccgaa acgtaagttg
      2050       2060       2070       2080       2090       2100
catgtctgtg gtaataaccc aacctgcgac ggttacgaga tcgaagaggg cgaattccgc
      2110       2120       2130       2140       2150       2160
attaaaggtt atgacggccc gatcgttgag tgtgaaaaat gtggctctga aatgcacctg
      2170       2180       2190       2200       2210       2220
aaaatggggc gtttcggtaa atacatggcc tgcaccaacg aagagtgtaa aaacacacgt
      2230       2240       2250       2260       2270       2280
aagattttac gtaacggcga agtggcacca ccgaaagaag atccggtgcc attacctgag
      2290       2300       2310       2320       2330       2340
ctgccgtgcg aaaaatcaga tgcttatttc gtgctgcgtg acggtgctgc cggtgtgttc
      2350       2360       2370       2380       2390       2400
ctggctgcca acactttccc gaaatcgcgt gaaacgcgtg cgccactggt ggaagagctt
      2410       2420       2430       2440       2450       2460
tatcgcttcc gcgaccgtct gccggaaaaa ctgcgttatc tggccgatgc gccacagcag
      2470       2480       2490       2500       2510       2520
gatccggaag gtaataagac catggttcgc tttagccgta aaaccaaaca gcaatatgtc
      2530       2540       2550       2560       2570       2580
tcttcggaaa aagacggaaa ggcgactggc tggtcagcat tttatgttga tggcaaatgg
      2590
gttgaaggaa aaaaataa    

Last modification date: Oct. 5, 2011