O2-methyl T (O2meT)

FULL NAME: O2-methylthymine


DESCRIPTION:
O2-methylcytosine (O2MeC) and O2-methylthymine (O2MeT) are minor reaction products formed by treatment of DNA with alkylating agents such as MNU or MNNG. Both lesions are repaired in vitro by E.coli AlkA glycosylase. O2MeC and O2MeT are predicted to interfere with minor groove contacts; yet, there have been very few studies of these modifications, making the lesions good candidates for future study. REACTION: alkylated DNA + H2O = 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA BRENDA id=14741

DAMAGE TYPE: methylation damage


DNA DAMAGE SOURCE(S) (MAIN):
N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)
SN1 methylating agents
N-methyl-N-nitrosourea (NMU)
alkylating agents


DNA DAMAGE EFFECT(S) (MAIN):
cytotoxic


DNA DAMAGE EFFECT(S) (MINOR):
substitution


PATHWAYS:
base excision repair (BER)


DNA repair protein(s) related to damage:
AlkA


Last modification date: Nov. 8, 2011