1,N2-etheno-G (1,2εG) in dsDNA

FULL NAME: 1,N2-ethenoguanine in dsDNA


DESCRIPTION:
1,N2-ethenoguanine (1,2-εG) and its isomer 3,N2-ethenoguanine (2,3-εG) are cyclic DNA adducts formed, as with eA, by reagents such as chloroacetylaldehyde or 4-hydroperoxy-2-nonenal. Significantly, the former has been found in the liver DNA of rodents exposed to vinyl chloride. 1,2-eG can moderately block DNA polymerase and cause G → T and G → C base substitutions, as well as frameshift mutations. It can be repaired by mammalian uracil-DNA glycosylase (MUG) and AAG. 1,2-εG was shown to be repaired, albeit weakly, by BER using a truncated form of the AAG enzyme.

DAMAGE TYPE: DNA adduct


DNA DAMAGE SOURCE(S) (MAIN):
lipid peroxidation (LPO)
vinyl chloride metabolites
chloroacetaldehyde (CAA)


DNA DAMAGE EFFECT(S) (MAIN):
-1 frameshift
deletion
G→C transversion
G→T transversion
mutagenesis
point mutation
stalled replication fork
transversion


DNA DAMAGE EFFECT(S) (MINOR):
-1 frameshift
G→C transversion
G→T transversion
mutagenesis
point mutation
transversion


PATHWAYS:
base excision repair (BER)


DNA repair protein(s) related to damage:
Mug
ANPG (MPG)
Mug


Last modification date: Oct. 12, 2011